DNA and protein synthesis inhibition in chinese hamster ovary cells by dichlorophenoxyacetic acid
| Autor: | Héctor Balegno, Viviana Rivarola, Jorge R. Bergesse |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
2
4-Dichlorophenoxyacetic acid Biology Toxicology chemistry.chemical_compound Cricetulus Cricetinae Protein biosynthesis Animals Hydroxyurea Cells Cultured Confluency DNA synthesis Chinese hamster ovary cell Cell Cycle Ovary DNA General Medicine Cell cycle Molecular biology chemistry Biochemistry Protein Biosynthesis Female 2 4-Dichlorophenoxyacetic Acid Thymidine |
| Zdroj: | Toxicology Letters. 29:137-144 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/0378-4274(85)90034-7 |
| Popis: | The effects of the herbicide dichlorophenoxyacetic acid (2,4-D) on DNA and protein synthesis were investigated in chinese hamster ovary cells (CHO) employing two different methods. The results showed that the herbicide affects DNA and protein synthesis depending on the stage of growth and method of treatment. 2,4-D action appears to concentrate the cells mainly in the G1/S boundary of the cell cycle. The effect is expressed as an inhibition of DNA and protein synthesis. This effect was revealed not only by the chemical determination of DNA and protein synthesis but also by experiments using autoradiography, using the labelling index to detect the incorporation of [3H]thymidine into the cells. Labelling of the cell nucleus was reduced markedly when cells treated with 2,4-D were in confluency for 4 days after reaching plateau growth. |
| Databáze: | OpenAIRE |
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