A novel carbohydrate-binding surface layer protein from the hyperthermophilic archaeon Pyrococcus horikoshii
| Autor: | Tomoyuki Koga, Toshifumi Ueda, Shuichiro Goda, Ryo Kuriura, Kenichiro Yamashita |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Protein Denaturation Hot Temperature Protein Conformation Archaeal Proteins Carbohydrate binding Green Fluorescent Proteins Calorimetry medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Chromatography Affinity Acetylglucosamine Genes Archaeal Micrococcus Analytical Chemistry 03 medical and health sciences Pyrococcus horikoshii X-Ray Diffraction Affinity chromatography Scattering Small Angle Escherichia coli medicine Amino Acid Sequence Cloning Molecular Molecular Biology biology Molecular mass Protein Stability Chemistry Organic Chemistry Surface-layer protein Isothermal titration calorimetry General Medicine biology.organism_classification Archaea Agglutination (biology) 030104 developmental biology Electrophoresis Polyacrylamide Gel Cell envelope Bacteria Protein Binding Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 82(8):1327-1334 |
| ISSN: | 0916-8451 1327-1334 |
| Popis: | In Archaea and Bacteria, surface layer (S-layer) proteins form the cell envelope and are involved in cell protection. In the present study, a putative S-layer protein was purified from the crude extract of Pyrococcus horikoshii using affinity chromatography. The S-layer gene was cloned and expressed in Escherichia coli. Isothermal titration calorimetry analyses showed that the S-layer protein bound N- acetylglucosamine and induced agglutination of the gram-positive bacterium Micrococcus lysodeikticus. The protein comprised a 21-mer structure, with a molecular mass of 1,340 kDa, as determined using small-angle X-ray scattering. This protein showed high thermal stability, with a midpoint of thermal denaturation of 79 °C in dynamic light scattering experiments. This is the first description of the carbohydrate-binding archaeal S-layer protein and its characteristics. Bioscience, Biotechnology and Biochemistry, 82(8), pp.1327-1334; 2018 |
| Databáze: | OpenAIRE |
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