Deciphering the Complexity of Sainfoin (Onobrychis viciifolia) Proanthocyanidins by MALDI-TOF Mass Spectrometry with a Judicious Choice of Isotope Patterns and Matrixes
| Autor: | Elisabetta Stringano, Irene Mueller-Harvey, Trevor Gibson, Celia Smith, Wayne Hayes, Rainer Cramer |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Glycosylation
Chromatography biology Isotope Chemistry Dimer Onobrychis viciifolia Acetophenones Fabaceae MALDI-TOF Mass Spectrometry Mass spectrometry biology.organism_classification Analytical Chemistry Adduct Matrix-assisted laser desorption/ionization chemistry.chemical_compound Proanthocyanidin Isotope Labeling Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Proanthocyanidins Dimerization |
| Zdroj: | Analytical Chemistry. 83:4147-4153 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Use of superdihydroxybenzoic acid as the matrix enabled the analysis of highly complex mixtures of proanthocyanidins from sainfoin (Onobrychis viciifolia) by MALDI-TOF mass spectrometry. Proanthocyanidins contained predominantly B-type homopolymers and heteropolymers up to 12-mers (3400 Da). Use of another matrix, 2,6-dihydroxyacetophenone, revealed the presence of A-type glycosylated dimers. In addition, we report here how a comparison of the isotopic adduct patterns, which resulted from Li and Na salts as MALDI matrix additives, could be used to confirm the presence of A-type linkages in complex proanthocyanidin mixtures. Preliminary evidence suggested the presence of A-type dimers in glycosylated prodelphinidins and in tetrameric procyanidins and prodelphinidins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|