LncRNA H19 suppresses pyroptosis of cardiomyocytes to attenuate myocardial infarction in a PBX3/CYP1B1-dependent manner
| Autor: | Youjian Han, Meijuan Chen, Bo Dong, Chanjiao Yao |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Clinical Biochemistry Cell Myocardial Infarction Down-Regulation Apoptosis Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Genes Reporter Cell Line Tumor Proto-Oncogene Proteins Pyroptosis medicine Animals Humans Myocytes Cardiac Hypoxia Molecular Biology Cell Proliferation Homeodomain Proteins Gene knockdown Reporter gene Cell growth Chemistry Cell Biology General Medicine Transfection female genital diseases and pregnancy complications Rats body regions 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cytochrome P-450 CYP1B1 embryonic structures Cancer research RNA Long Noncoding HeLa Cells Signal Transduction |
| Zdroj: | Molecular and Cellular Biochemistry. 476:1387-1400 |
| ISSN: | 1573-4919 0300-8177 |
| DOI: | 10.1007/s11010-020-03998-y |
| Popis: | Myocardial infarction (MI) is a major cause of cardiovascular disease which poses great healthy and financial burden for individuals. MI can be mainly induced by hypoxia. Therefore, in this study, we aimed to explore the function and mechanism of lncRNA H19 on hypoxia-induced pyroptosis of cardiomyocytes. Peripheral blood from healthy controls and MI patients was collected for determination of mRNA and protein expression levels of H19 and CYP1B1. The correlation between these two factors was analyzed. Then MI rat model was established and injected with H19 overexpression/CYP1B1 knockdown plasmid, in which the infraction area and pathological morphology were observed. Hypoxic cardiomyocytes were transfected with overexpression or knockdown of H19 and CYP1B1 for determination of NLRP3, ASC, caspase-1, IL-1β, IL-18, CyclinD1, and PCNA. Cell proliferation ability was assessed by CCK8. RIP and dual luciferase gene reporter assay were applied to verify the binding among H19, PBX3 and CYP1B1. Downregulated H19 and upregulated CYP1B1 were observed in MI patients. A negative correlation was found for H19 and CYP1B1 expressions. Transfection of H19 overexpression or CYP1B1 knockdown could attenuate the MI progression in MI rats. In hypoxic cardiomyocytes, H19 overexpression or CYP1B1 knockdown could also inhibit NLRP3, ASC, caspase-1, IL-1β, and IL-18 in addition to suppressing cell apoptosis rate and promoting cell proliferation rate. Different expression pattern was found in cells transfected with H19 knockdown or CYP1B1 overexpression. Overexpression of CYP1B1 could abrogate the suppressive effect of H19 on pyroptosis of cardiomyocytes. H19 could inhibit activity of CYP1B1 promoters by regulating PBX3. H19 could inhibit CYP1B1 expression in a PBX3-dependent way and thus attenuate cell pyroptosis of cardiomyocytes. |
| Databáze: | OpenAIRE |
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