Characterization and Incompatibility Properties of ROM- Derivatives of pBR322-based Plasmids
| Autor: | William C.A. Tacon, Terence J. Smith, Marilyn E. Nugent |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
DNA
Bacterial Electrophoresis Agar Gel Genetics Mutation ColE1 Base Sequence Mutant Chromosomes Bacterial Biology medicine.disease_cause Microbiology Molecular biology PBR322 RNA Bacterial chemistry.chemical_compound Plasmid chemistry Escherichia coli medicine Nucleic Acid Conformation Coding region Transversion DNA Plasmids |
| Zdroj: | Microbiology. 132:1021-1026 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-132-4-1021 |
| Popis: | Summary: We have characterized a copy number mutant of the pBR322-based plasmid pWT111. A single nucleotide transversion in loop II’ of RNAI results in an eightfold increase in plasmid copy number. Removal of the rom coding region from pWT111cop results in a further sixfold increase in copy number. We present evidence that ROM is involved in the strong incompatibility effect seen between pMB1 and ColE1 type plasmids. |
| Databáze: | OpenAIRE |
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