Laboratory vector competence experiments with yellow fever virus and five South African mosquito species including Aedes aegypti
| Autor: | Alan Kemp, P. G. Jupp |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Veterinary medicine
Population Aedes aegypti Virus South Africa Flaviviridae Aedes Risk Factors Yellow Fever medicine Animals Humans education Infectivity education.field_of_study biology Yellow fever Public Health Environmental and Occupational Health Haplorhini General Medicine biology.organism_classification medicine.disease Virology Insect Vectors Flavivirus Infectious Diseases Parasitology Furcifer |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 96:493-498 |
| ISSN: | 0035-9203 |
| DOI: | 10.1016/s0035-9203(02)90417-7 |
| Popis: | Three domestic and peridomestic mosquito species, selected because their prevalence, distribution and ecology favoured them as potential urban vectors of yellow fever (YF) in South Africa, were submitted to numerous tests for infectivity [measured as dose needed to infect 50% of the mosquitoes (MID50)], mainly with a Kenyan strain (BC7914) of the virus. Use of a Nigerian virus strain (TVP1617) did not significantly alter infectivity. After artificial infective blood meals with titres of 7 · 0–8 · 0 log10MID50/mL, head squash infection rates (HSIRs) determined by the indirect fluorescent antibody test were 0–4% (Eretmapodites quinquevittatus), 0–29% (Aedes simpsoni s. s.) and 0–21% (5 populations of Aedes aegypti). For some populations of Ae. aegypti tests were repeated with blood meals incorporating freshly prepared rather than frozen mouse brain but HSIRs did not increase. HSIRs did increase when a high infecting titre of 9 · 0 log10MID50/mL was used with the Richards Bay population (67–90%). It is concluded that these 3 mosquito species are potentially poor YF vectors but that Ae. simpsoni and Richards Bay Ae. aegypti are the most susceptible to the virus. However, the latter 2 species could support person-to-person transmission only if they were present at very high densities. This rarely occurs with Ae. simpsoni in South Africa but Ae. aegypti may occur at high densities although only in discrete foci. The feral Ae. furcifer and Ae. cordellieri had HSIRs of 29% and 3% respectively and Ae. furcifer ‘transmitted’ the virus in vitro at a transmission rate of 25%. This suggests that Ae. furcifer would be more important than Ae. cordellieri in transmission between monkeys in West Africa. |
| Databáze: | OpenAIRE |
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