Estimating telomere length from whole genome sequence data
| Autor: | Eleftheria Zeggini, Richard Durbin, Kirsten Kelly, David Collier, Robert Kenneth Semple, Sadaf Farooqi, Louise Gallagher, Massimo Mangino, Zhihao Ding, Peter Scambler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Genome Restriction fragment Telomere Homeostasis Genetics Leukocytes Humans Exome Southern blot Aged Repetitive Sequences Nucleic Acid Whole genome sequencing biology Base Sequence Models Genetic Genome Human Sequence Analysis DNA Middle Aged Telomere Cross-Sectional Studies biology.protein Methods Online Human genome Software |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs. |
| Databáze: | OpenAIRE |
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