Studies of odd bases in yeast mitochondrial tRNA: Absence of the fluorescent 'Y' base in mitochondrial DNA coded tRNAPhe, absence of 4-thiouridine
| Autor: | G. Dirheimer, A.J.C. Stahl, R. Martin, J.M. Schneller |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Cytoplasm
Mitochondrial DNA Phenylalanine Thiouridine Saccharomyces cerevisiae Biophysics DNA Mitochondrial Biochemistry RNA Transfer Escherichia coli Base (exponentiation) Molecular Biology Chromatography biology Nucleic Acid Hybridization Cell Biology biology.organism_classification Fluorescence Molecular biology Yeast Mitochondria Spectrometry Fluorescence ATP–ADP translocase |
| Zdroj: | Biochemical and Biophysical Research Communications. 64:1046-1053 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(75)90153-9 |
| Popis: | Reversed phase chromatography of mitochondrial [3H] Phe-tRNA from Saccharomyces cerevisiae shows only one peak which elutes distinctly from cytoplasmic [14C] Phe-tRNA. Mitochondrial tRNAPhe from this peak hybridizes specifically with ϱ+ and a ϱ− mitochondrial DNA. Search for rare bases in mitochondrial tRNA shows the absence of the eukaryotic “Y” base and of the prokariotic s4U base. |
| Databáze: | OpenAIRE |
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