Rap1, AF6 and Pak4 cooperate with Par3 to promoteZAassembly and remodeling in the fly photoreceptor
| Autor: | Rhian F Walther, Noelia Pinal, Franck Pichaud, Clare Rogerson, Mubarik Burki |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0303 health sciences
animal structures Epithelial cell morphogenesis Kinase Morphogenesis Biology Cell biology Adherens junction enzymes and coenzymes (carbohydrates) 03 medical and health sciences 0302 clinical medicine embryonic structures Cancer research Phosphorylation Compartment (development) Rap1 Small GTPase 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/155945 |
| Popis: | The epithelialZonula adherens (ZA)is a main adhesion compartment that enables organogenesis by allowing epithelial cells to assemble into sheets. HowZAassembly is regulated during epithelial cell morphogenesis is not fully understood. We show that duringZAmorphogenesis, the function of the small GTPase Rap1 and the F-actin binding protein AF6/Cno are both linked to that of the P21-activated kinase Pak4/Mbt. We find that Rap1 and Mbt regulate each other’s localization at theZAand cooperate in promoting ECadherin stabilization. During this process Cno regulates the recruitment of Baz at theZA,a process that is also regulated by Arm phosphorylation by Mbt. Altogether, we propose that Rap1, Cno and Mbt regulateZAmorphogenesis by coordinating ECadherin stabilization and Baz recruitment and retention. In addition, our work uncovers a new link between two main oncogenes, Rap1 and Pak4/Mbt, in a model developing epithelial cell. |
| Databáze: | OpenAIRE |
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