The Babesia bovis merozoite surface antigen 1 hypervariable region induces surface-reactive antibodies that block merozoite invasion
| Autor: | Shawn J. Berens, Terry F. McElwain, Tanya LeRoith, Stephen A. Hines, Kelly A. Brayton, Junzo Norimine, Wendy C. Brown |
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| Rok vydání: | 2006 |
| Předmět: |
medicine.drug_class
Immunology Molecular Sequence Data Antibodies Protozoan Monoclonal antibody Microbiology law.invention Apicomplexa Antigen stomatognathic system law parasitic diseases medicine Animals Amino Acid Sequence Merozoite Surface Protein 1 Infectivity biology Antibodies Monoclonal Babesia bovis biology.organism_classification Virology Molecular biology Hypervariable region nervous system diseases Infectious Diseases Recombinant DNA biology.protein Parasitology Antibody Fungal and Parasitic Infections |
| Zdroj: | Infection and immunity. 74(6) |
| ISSN: | 0019-9567 |
| Popis: | A hypervariable region (HVR) previously identified in the carboxy-terminal one-third of the Babesia bovis variable merozoite surface antigen family was more extensively analyzed in merozoite surface antigen 1 (MSA-1) from 16 strains and isolates. The MSA-1 HVR is proline rich and contains three semiconserved motifs nearly identical to those described for the related family member MSA-2. Two MSA-1-specific monoclonal antibodies previously shown to be reactive with the merozoite surface bound to a recombinant construct encoding the HVR, indicating that the HVR is surface exposed and accessible to antibody binding. Importantly, these surface-reactive, HVR-specific monoclonal antibodies were capable of inhibiting merozoite infectivity of the host erythrocyte in vivo. The results indicate that the MSA-1 HVR is involved in erythrocyte invasion and suggest that selection of MSA-1 variants may be driven by invasion-blocking antibodies. |
| Databáze: | OpenAIRE |
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