Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo
| Autor: | Concepcion Rodriguez Esteban, Weizhi Ji, Hong Wang, Tianqing Li, Chenyang Si, May Schwarz, Ran Zhu, Honglian Shao, Llanos Martinez Martinez, Juan Carlos Izpisua Belmonte, Nianqin Sun, Pengpeng Yang, Hongkui Deng, Shao-Xing Dai, Wei Si, Estrella Nuñez Delicado, Yu Kang, Youyue Zhang, Yuyu Niu, Zongyong Ai, Jun Wu, E. Zhang, Zhenzhen Chen, Tao Tan, Reyna Hernández-Benítez, W. Travis Berggren |
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| Rok vydání: | 2020 |
| Předmět: |
Pluripotent Stem Cells
Cell Biology Regenerative medicine Chimerism General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Chimera (genetics) 0302 clinical medicine medicine Animals Humans Cell Lineage RNA-Seq Induced pluripotent stem cell Cells Cultured 030304 developmental biology 0303 health sciences Embryo Cell Differentiation Embryo Mammalian Cell biology Transplantation Crosstalk (biology) Macaca fascicularis medicine.anatomical_structure Blastocyst Female Single-Cell Analysis Transcriptome 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | Cell. 184(8) |
| ISSN: | 1097-4172 |
| Popis: | Summary Interspecies chimera formation with human pluripotent stem cells (hPSCs) represents a necessary alternative to evaluate hPSC pluripotency in vivo and might constitute a promising strategy for various regenerative medicine applications, including the generation of organs and tissues for transplantation. Studies using mouse and pig embryos suggest that hPSCs do not robustly contribute to chimera formation in species evolutionarily distant to humans. We studied the chimeric competency of human extended pluripotent stem cells (hEPSCs) in cynomolgus monkey (Macaca fascicularis) embryos cultured ex vivo. We demonstrate that hEPSCs survived, proliferated, and generated several peri- and early post-implantation cell lineages inside monkey embryos. We also uncovered signaling events underlying interspecific crosstalk that may help shape the unique developmental trajectories of human and monkey cells within chimeric embryos. These results may help to better understand early human development and primate evolution and develop strategies to improve human chimerism in evolutionarily distant species. |
| Databáze: | OpenAIRE |
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