Plakoglobin Reduces the in vitro Growth, Migration and Invasion of Ovarian Cancer Cells Expressing N-Cadherin and Mutant p53

Autor: Manijeh Pasdar, Ghazal Danesh, Mahsa Alaee
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Carcinoma Cells
Gene Expression
lcsh:Medicine
Biochemistry
Metastasis
0302 clinical medicine
Cell Movement
Medicine and Health Sciences
lcsh:Science
Ovarian Neoplasms
Cultured Tumor Cells
Multidisciplinary
Cell migration
Cadherins
Precipitation Techniques
Ovarian Cancer
Cell Motility
Oncology
030220 oncology & carcinogenesis
Gene Knockdown Techniques
Female
Biological Cultures
Research Article
Immunoblotting
Plakoglobin
Molecular Probe Techniques
Cell Migration
Biology
Research and Analysis Methods
03 medical and health sciences
Antigens
CD
Cell Line
Tumor
DNA-binding proteins
medicine
Cell Adhesion
Genetics
Humans
Immunoprecipitation
Vimentin
Metastasis suppressor
Neoplasm Invasiveness
Gene Regulation
Molecular Biology Techniques
Molecular Biology
Cell Proliferation
Cadherin
Cell growth
Tumor Suppressor Proteins
lcsh:R
Cancers and Neoplasms
Biology and Life Sciences
Proteins
Cell Biology
Cell Cultures
medicine.disease
Regulatory Proteins
Cytoskeletal Proteins
030104 developmental biology
Desmoplakins
Catenin
Cancer research
Mutant Proteins
lcsh:Q
gamma Catenin
Tumor Suppressor Protein p53
Ovarian cancer
Gynecological Tumors
Transcription Factors
Developmental Biology
Zdroj: PLoS ONE, Vol 11, Iss 5, p e0154323 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: Aberrant expression of cadherins and catenins plays pivotal roles in ovarian cancer development and progression. Plakoglobin (PG, γ-catenin) is a paralog of β-catenin with dual adhesive and signaling functions. While β-catenin has known oncogenic function, PG generally acts as a tumor/metastasis suppressor. We recently showed that PG interacted with p53 and that its growth/metastasis inhibitory function may be mediated by this interaction. Very little is known about the role of PG in ovarian cancer. Here, we investigated the in vitro tumor/metastasis suppressor effects of PG in ovarian cancer cell lines with mutant p53 expression and different cadherin profiles. We showed that the N-cadherin expressing and E-cadherin and PG deficient ES-2 cells were highly migratory and invasive, whereas OV-90 cells that express E-cadherin, PG and very little/no N-cadherin were not. Exogenous expression of PG or E-cadherin or N-cadherin knockdown in ES-2 cells (ES-2-E-cad, ES-2-PG and ES-2-shN-cad) significantly reduced their migration and invasion. Also, PG expression or N-cadherin knockdown significantly decreased ES-2 cells growth. Furthermore, PG interacted with both cadherins and with wild type and mutant p53 in normal ovarian and ES-2-PG cell lines, respectively.
Databáze: OpenAIRE
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