The Effect of Osteopontin on the Adhesion of Calcium Oxalate Crystals to Madin-Darby Canine Kidney Cells
| Autor: | T, Yamate, K, Kohri, T, Umekawa, N, Amasaki, Y, Isikawa, M, Iguchi, T, Kurita |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Sialoglycoproteins Urology Calcium oxalate chemistry.chemical_element Calcium Kidney chemistry.chemical_compound Dogs Thrombin stomatognathic system Internal medicine Cell Adhesion medicine Animals Osteopontin Cell adhesion Cells Cultured Calcium Oxalate biology urogenital system business.industry Adhesion Phosphoproteins Molecular biology Endocrinology medicine.anatomical_structure chemistry Cell culture Microscopy Electron Scanning biology.protein Urinary Calculi Crystallization business medicine.drug |
| Zdroj: | European Urology. 30:388-393 |
| ISSN: | 1873-7560 0302-2838 |
| DOI: | 10.1159/000474201 |
| Popis: | Objective : We have recently reported that osteopontin protein (OPN) is present in calcium-containing urinary calculi and in rat distal tubular cells and that it is an important substance of the matrix in urinary stone formation. To investigate how OPN participates in urinary stone formation, we assessed the role of OPN in the adhesion of the surfaces of Madin-Darby canine kidney (MDCK) cells, which are distal tubular cells of the dog, to calcium oxalate crystals. Methods : We cultured MDCK cells with OPN, thrombin, which is an OPN inhibitor, Arg-Gly-Asp peptide and Arg-Gly-Glu peptide as a control for Arg-Gly-Asp peptide, and examined changes in adhesion of calcium oxalate crystals to the cells by scanning electron microscopy and the calcium ( 45 Ca) concentration around the cells. Results : The 45 Ca concentration in calcium oxalate crystals adhering to MDCK cells was about 1.4 times higher in MDCK cells incubated with OPN than in an MDCK-cell-negative group, and was about one half that in MDCK cells incubated with thrombin than in the group without MDCK cells. Conclusion : OPN is considered to be an accelerator of urinary stone formation because of the increase in adhesion of calcium oxalate crystals to the renal distal tubular epithelium in the presence of OPN. These findings suggest that OPN plays an important role in the formation of calcium-containing urinary stones. |
| Databáze: | OpenAIRE |
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