Increased plin2 expression in human skeletal muscle is associated with sarcopenia and muscle weakness
| Autor: | Marco Narici, Laura Barberi, Olivier R. Seynnes, Maria Conte, Stefano Salvioli, Daniela Caporossi, Ermanno Martucci, Miriam Capri, Andrea B. Maier, Antonio Musarò, Elena Bellavista, Alessio Degiovanni, Antonia D’Errico-Grigioni, Giovanni Trisolino, Francesco Vasuri, Aurelia Santoro, Claudio Franceschi |
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| Přispěvatelé: | Conte M, Vasuri F, Trisolino G, Bellavista E, Santoro A, Degiovanni Alessio, Martucci E, D'Errico-Grigioni A, Caporossi D, Capri M, Maier AB, Seynnes O, Barberi L, Musarò A, Narici MV, Franceschi C, Salvioli S, Centro Interdipartimentale Galvani (CIG), Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Departmento of Experimental, Diagnostic and Specialty Medicine, Institute of Oncology and Transplant Pathology, Reconstructive Hip and Knee Joint Surgery, Istituto Ortopedico Rizzoli, Bologna, Department of Health Science, University of Rome 'Foro Italico', Leiden University Medical Center (LUMC), Norwegian School of Sport Sciences, Norwegian School of Sport Sciences = Norges idrettshøgskole [Oslo] (NIH), Department of Anatomy, Histology, Forensic Medicine and Orthopedic [Roma] (DAHFMO), Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], School of Graduate Entry Medicine and Health, University of Nottingham, UK (UON), Neuromechanics, AMS - Ageing and Morbidity |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Aging
Sarcopenia protein p53 Mice 0302 clinical medicine intramuscular lipid 80 and over lcsh:Science pathophysiology Aged 80 and over 0303 health sciences clinical article Muscle Weakness muscle proteins STORAGE Muscle tissue lipid storage medicine.medical_specialty vastus lateralis muscle Perilipin 2 PERILIPIN 2 complication EXERCISE Perilipin-2 animal tissue lipids 03 medical and health sciences FAT INFILTRATION Humans human Muscle Strength skeletal muscle muscle thickness OLDER-ADULTS protein expression mouse Aged P53 animal model lcsh:R Muscle weakness medicine.disease muscle aging Endocrinology quadriceps femoris muscle immobilization CELLS Perilipin leg disease lcsh:Q 030217 neurology & neurosurgery skeletal muscles ACTIVATED RECEPTOR-GAMMA fat droplet very elderly Adipose tissue lcsh:Medicine membrane protein animal Insulin-Like Growth Factor I Multidisciplinary INSULIN SENSITIVITY article Skeletal unclassified drug adipose tissue APOPTOSIS medicine.anatomical_structure female perilipin muscle mass IGF-1 Muscle ACTIVATED RECEPTOR-GAMMA INSULIN SENSITIVITY FAT INFILTRATION OLDER-ADULTS PERILIPIN 2 EXERCISE APOPTOSIS STORAGE CELLS P53 muscle analysis Intramuscular fat perilipin 2 somatomedin C triacylglycerol somatomedin C adipose tissue adult aged aging animal experiment controlled study human tissue male muscle function muscle strength muscle weakness nonhuman sarcopenia signal transduction adolescent metabolism pathology young adult Adolescent Adult Animals Membrane Proteins Muscle Skeletal Signal Transduction Tumor Suppressor Protein p53 Young Adult medicine.symptom Research Article muscle tissue Adolescent Biology Internal medicine medicine biopsy 030304 developmental biology Skeletal muscle biology.protein physical inactivity [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2012, 8 (8), pp.e73709. ⟨10.1371/journal.pone.0073709⟩ PLoS ONE; Vol 8 Conte, M, Vasuri, F, Trisolino, G, Bellavista, E, Santoro, A, Degiovanni, A, Martucci, E, D'Errico-Grigioni, A, Caporossi, D, Capri, M, Maier, A B, Seynnes, O, Barberi, L, Musarò, A, Narici, M V, Franceschi, C & Salvioli, S 2013, ' Increased Plin2 Expression in Human Skeletal Muscle Is Associated with Sarcopenia and Muscle Weakness ', PLoS ONE, vol. 8, no. 8, e73709 . https://doi.org/10.1371/journal.pone.0073709 PLoS ONE, Vol 8, Iss 8, p e73709 (2013) PLoS ONE, 8(8):e73709. Public Library of Science PLoS ONE, 8(8) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0073709⟩ |
| Popis: | International audience; Human aging is associated with a progressive loss of muscle mass and strength and a concomitant fat accumulation in form of inter-muscular adipose tissue, causing skeletal muscle function decline and immobilization. Fat accumulation can also occur as intra-muscular triglycerides (IMTG) deposition in lipid droplets, which are associated with perilipin proteins, such as Perilipin2 (Plin2). It is not known whether Plin2 expression changes with age and if this has consequences on muscle mass and strength. We studied the expression of Plin2 in the vastus lateralis (VL) muscle of both healthy subjects and patients affected by lower limb mobility limitation of different age. We found that Plin2 expression increases with age, this phenomenon being particularly evident in patients. Moreover, Plin2 expression is inversely correlated with quadriceps strength and VL thickness. To investigate the molecular mechanisms underpinning this phenomenon, we focused on IGF-1/p53 network/signalling pathway, involved in muscle physiology. We found that Plin2 expression strongly correlates with increased p53 activation and reduced IGF-1 expression. To confirm these observations made on humans, we studied mice overexpressing muscle-specific IGF-1, which are protected from sarcopenia. These mice resulted almost negative for the expression of Plin2 and p53 at two years of age. We conclude that fat deposition within skeletal muscle in form of Plin2-coated lipid droplets increases with age and is associated with decreased muscle strength and thickness, likely through an IGF-1- and p53-dependent mechanism. The data also suggest that excessive intramuscular fat accumulation could be the initial trigger for p53 activation and consequent loss of muscle mass and strength. |
| Databáze: | OpenAIRE |
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