PURIFICATION AND SEPARATION OF FOLLICLE STIMULATING HORMONE (FSH) AND LUTEINIZING HORMONE (LH) FROM HUMAN POSTMENOPAUSAL GONADOTROPHIN (HMG)
| Autor: | Bruno Lunenfeld, I. D'Alessio, A. F. Parlow, Aliza Eshkol, P. Donini, Puzzuoli D |
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| Rok vydání: | 1966 |
| Předmět: |
endocrine system
medicine.medical_specialty Chromatography biology business.industry Chemistry Endocrinology Diabetes and Metabolism Size-exclusion chromatography General Medicine Urine Gonadotropic cell Electrophoresis Follicle-stimulating hormone Endocrinology Internal medicine HMG-CoA reductase medicine biology.protein Bioassay business Luteinizing hormone hormones hormone substitutes and hormone antagonists |
| Zdroj: | Acta Endocrinologica. 52:169-185 |
| ISSN: | 1479-683X 0804-4643 |
| DOI: | 10.1530/acta.0.0520169 |
| Popis: | A new approach to the preparation of biologically pure FSH from HMG is described. The method entails selective binding of the LH to antibodies against HCG. The bound LH was separated from FSH by DEAE-C chromatography. An FSH preparation was obtained with a potency of 329.7 IU-FSH/mg and undetectable LH activity at the highest dose tested. This preparation, however, was not homogeneous in the immuno-electrophoretic analysis. The nature of the LH activity »unseparable« from FSH by physico-chemical procedures is discussed. |
| Databáze: | OpenAIRE |
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