PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury
| Autor: | Yumin Yang, Guicai Li, Luzhong Zhang, Yinxin Zhao, Shiyu Chen, Xiaoli Yan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
food.ingredient Alginates 0206 medical engineering Biomedical Engineering Acrylic Resins 02 engineering and technology Gelatin Biomaterials Masson's trichrome stain Rats Sprague-Dawley Electrical conduit food Tissue engineering Peripheral Nerve Injuries medicine Animals cardiovascular diseases Tissue Scaffolds Regeneration (biology) Metals and Alloys 021001 nanoscience & nanotechnology 020601 biomedical engineering Sciatic Nerve Nerve Regeneration Rats surgical procedures operative Peripheral nerve injury cardiovascular system Ceramics and Composites Female Graphite Sciatic nerve Swelling medicine.symptom 0210 nano-technology Oligopeptides Biomedical engineering |
| Zdroj: | Journal of biomedical materials research. Part A. 107(6) |
| ISSN: | 1552-4965 |
| Popis: | The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research. The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects. The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm. Then the conduit was further modified with YIGSR peptide. The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration. The results of diameter analysis indicated that the wall of hydrogel conduit was uniform. The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties. Further on, hematoxylin-eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve. Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1273-1283, 2019. |
| Databáze: | OpenAIRE |
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