Validation of the MycoAlign system for Mycobacterium spp. identification
| Autor: | Geoffrey Land, Patricia Cernoch, Celest Austin, Daniel H. Farkas, Randall J. Olsen, Edward A. Graviss |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Bacilli Chaperonins Genotype Molecular Sequence Data Mycobacterium law.invention Microbiology Identification system Bacterial Proteins law RNA Ribosomal 16S Identity score Humans Polymerase chain reaction biology Chaperonin 60 General Medicine Molecular diagnostics biology.organism_classification RNA Ribosomal 23S Infectious Diseases Identification (biology) Reagent Kits Diagnostic |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 59:105-108 |
| ISSN: | 0732-8893 |
| Popis: | Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)-positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81-87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls. |
| Databáze: | OpenAIRE |
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