In vivo anti-inflammatory activity of Fabaceae species extracts screened by a new ex vivo assay using human whole blood
| Autor: | Daniela Aparecida Chagas-Paula, Patricia Sartorelli, Olívia da Silva Domingos, João Henrique G. Lago, Danielle Ferreira Dias, Welton Rosa, Marisi G. Soares, Michael Murgu, Paula Pio de Oliveira Salem, Ivo Santana Caldas |
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| Rok vydání: | 2020 |
| Předmět: |
Electrospray
Spectrometry Mass Electrospray Ionization Lipopolysaccharide medicine.drug_class Anti-Inflammatory Agents Plant Science Tandem mass spectrometry 01 natural sciences Biochemistry Anti-inflammatory Analytical Chemistry chemistry.chemical_compound In vivo Tandem Mass Spectrometry Drug Discovery medicine Humans Chromatography High Pressure Liquid Whole blood Plant Extracts 010401 analytical chemistry Fabaceae General Medicine 0104 chemical sciences 010404 medicinal & biomolecular chemistry Complementary and alternative medicine chemistry Molecular Medicine Biological Assay Ex vivo Food Science Chromatography Liquid |
| Zdroj: | Phytochemical analysis : PCAREFERENCES. 32(5) |
| ISSN: | 1099-1565 |
| Popis: | INTRODUCTION Plants have been considered a promising source for discovering new compounds with pharmacological activities. The Fabaceae family comprises a large variety of species that produce substances with diverse therapeutic potential, including anti-inflammatory activity. The limitations of current anti-inflammatories generate the need to research new anti-inflammatory structures with higher efficacy as well as develop methods for screening multiple samples, reliably and ethically, to assess such therapeutic properties. OBJECTIVE Validate and apply a quantification method for prostaglandin E2 (PGE2 ) production from an ex vivo assay in human blood in order to screen anti-inflammatory activity present in many Fabaceae species extracts. METHODS Human blood was incubated with extracts from 47 Fabaceae species. After lipopolysaccharide (LPS)-induced inflammation, PGE2 was quantified in the plasma by liquid chromatography with tandem mass spectrometry (LC-MS/MS). The extracts that presented PGE2 production inhibition were further assessed through in vivo assay and then chemically characterised through an analysis of ultra-performance liquid chromatography electrospray ionisation quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-QTOF-MS2 ) data. RESULTS The new ex vivo anti-inflammatory assay showed that five out of the 47 Fabaceae species inhibited PGE2 production. Results from an in vivo assay and the metabolic profile of the active extracts supported the anti-inflammatory potential of four species. CONCLUSION The quantification method for PGE2 demonstrated fast, sensitive, precise, and accurate results. The new ex vivo anti-inflammatory assay comprised a great, reliable, and ethical approach for the screening of a large number of samples before an in vivo bioassay. Additionally, the four active extracts in both ex vivo and in vivo assays may be useful for the development of more efficient anti-inflammatory drugs. |
| Databáze: | OpenAIRE |
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