TaAbc1, a member of Abc1-like family involved in hypersensitive response against the stripe rust fungal pathogen in wheat
| Autor: | Xiaojie Wang, Xiaojing Wang, Zhensheng Kang, Li Huang, Yinghui Duan, Shuining Yin, Hongchang Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Hypersensitive response
Transcription Genetic Plant Cell Biology Plant Pathogens Gene Expression lcsh:Medicine Plant Science Biology Biochemistry Chloroplast Gene Expression Regulation Plant RNA interference Molecular Cell Biology Gene Order Gene expression Genetics Hypersensitivity Gene family Cloning Molecular lcsh:Science Pathogen Gene Triticum Plant Diseases Multidisciplinary Abiotic stress Basidiomycota lcsh:R Proteins food and beverages Plant Pathology Phenotype Transmembrane Proteins ATP-Binding Cassette Transporters RNA Interference lcsh:Q Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 3, p e58969 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | To search for genes involved in wheat (Triticum aestivum L.) defense response to the infection of stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst), we identified and cloned a new wheat gene similar to the genes in the Abc1-like gene family. The new gene, designated as TaAbc1, encodes a 717-amino acid, 80.35 kD protein. The TaAbc1 protein contains two conserved domains shared by Abc1-like proteins, two trans-membrane domains at the C-terminal, and a 36-amino acid chloroplast targeting presequence at the N-terminal. Characterization of TaAbc1 expression revealed that gene expression was tissue-specific and could be up-regulated by biotic agents (e.g., stripe rust pathogen) and/or by an abiotic stress like wounding. High-fold induction was associated with the hypersensitive response (HR) triggered only by avirulent stripe rust pathotypes, suggesting that TaAbc1 is a rust-pathotype specific HR-mediator. Down-regulating TaAbc1 reduced HR but not the overall resistance level in Suwon11 to CYR23, suggesting TaAbc1 was involved in HR against stripe rust, but overall host resistance is not HR-dependent. |
| Databáze: | OpenAIRE |
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