Interaction of arylsulfatases A and B with maspin: A possible explanation for dysregulation of tumor cell metabolism and invasive potential of colorectal cancer
| Autor: | Ioan Jung, Zsolt Kovacs, Laura Banias, Simona Gurzu, Krisztina Szalman, Tivadar Bara |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
ARSB gene
Arylsulfatase Tissue biology Colorectal cancer business.industry Maspin Tumor cells General Medicine Arylsulfatases Metabolism medicine.disease 03 medical and health sciences Blood 0302 clinical medicine 030220 oncology & carcinogenesis Cancer research biology.protein medicine Prospective Study 030211 gastroenterology & hepatology business |
| Zdroj: | World Journal of Clinical Cases |
| ISSN: | 2307-8960 |
| DOI: | 10.12998/wjcc.v7.i23.3990 |
| Popis: | BACKGROUND Although it has been shown that arylsulfatases are lost in colorectal cancer (CRC) cell lines, their exact role in the carcinogenesis and behavior of this cancer was not elucidated. No data about the correlation between serum and immunohistochemical (IHC) level of arylsulfatases (ARSA, ARSB) in patients with CRC were published yet. AIM To evaluate the possible prognostic value of ARSA and/or ARSB in CRC, at circulating and protein levels. METHODS The present study included 45 consecutive patients who were prospectively diagnosed with CRC. For IHC stains (protein expression) ARSA, ARSB and maspin expression were quantified. For these markers, cytoplasmic expression was taken into account. For gene expression study, circulating mRNA was isolated from all patients, before surgery. A group of 45 healthy patients without inflammatory or tumor pathologies was used as control group. Reverse transcription and Taqman Gene Expression Array were used for ARSB gene expression. RESULTS The preoperative circulating RNA level of the ARSB gene was significantly decreased in patients with CRC (RQ < 1), compared with the control group (RQ > 1). A more significant decrease (RQ < 0.5) occurred in ulcero-infiltrative maspin-positive adenocarcinomas, with a higher degree of tumor budding, diagnosed in locally advanced stages (pT3/4). ARSA/maspin immunopositivity indicated a higher risk for lymph node metastasis, while triple positivity for maspin/ARSA/ARSB and ARSB gene expression level < 0.5 were indicators of CRC aggressive behavior, independent of lymph node status. CONCLUSION The significant independent negative prognostic factors of CRC are the ulcero-infiltrative aspect, high budding degree, triple positivity for maspin, ARSA and ARSB, and low ARSB gene expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|