Use of fluorescent lectin binding to distinguish eggs of gastrointestinal nematode parasites of sheep
| Autor: | Heather V. Simpson, L.W. McMurtry, Saleh Umair, J.S. Knight |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Peanut agglutinin Nematoda Carbohydrates Sheep Diseases Polymerase Chain Reaction Fluorescence Microbiology Specimen Handling 03 medical and health sciences Feces Agglutinin Species Specificity Lectins parasitic diseases Botany Animals Trichostrongylus Nematode Infections Ovum Sheep General Veterinary biology Temperature Lectin General Medicine 030108 mycology & parasitology biology.organism_classification Wheat germ agglutinin Nematode biology.protein Parasitology Nematodirus Haemonchus contortus Protein Binding |
| Zdroj: | Veterinary parasitology. 217 |
| ISSN: | 1873-2550 |
| Popis: | The binding of a panel of 19 lectins to carbohydrates on the eggs of economically important nematode parasites of sheep has been assessed as the basis of a rapid test to distinguish parasite eggs, at least at the genus level. A total of six lectins can be used to identify eggs of six nematode parasites: peanut agglutinin (PNA) for Haemonchus contortus; Lens culinaris agglutinin (LCA) for Teladorsagia sp; Aleuria aurantia agglutinin (AAL) for Trichostrongylus sp; Psophocarpus tetragonolobus‑II (PTLII) for Nematodirus sp; Lotus tetragonolobus lectin (LTL) for Cooperia sp and wheat germ agglutinin (WGA) for Chabertia ovina. For WGA, LCA and LTL, weak binding was also observed to H. contortus and Teladorsagia sp, Trichostrongylus sp and C. ovina eggs, respectively. Nematode eggs in two faecal samples were identically identified by both lectin binding and PCR, except for PCR identification of the eggs of Nematodirus sp, as these did not lyse. Lectins bound best to H. contortus eggs extracted from fresh faecal samples or after storage at room temperature or 4 °C for up to 24 h, but eggs stored at -20 °C or -80 °C did not bind PNA. |
| Databáze: | OpenAIRE |
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