Binding of Mucin by E. coli from Human Gut
| Autor: | G. Yu. Lomakina, N. G. Balabushevich, Olga Pobeguts, T. V. Vakhrusheva, M A Moshkovskaya, E. V. Mikhalchik, Yu. P. Baikova, P L Shcherbakov, E A Sholina, S. A. Gusev |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
02 engineering and technology Absorption (skin) Bacterial Adhesion General Biochemistry Genetics and Molecular Biology law.invention Feces 03 medical and health sciences Crohn Disease Dry weight Confocal microscopy law Escherichia coli Extracellular Humans Incubation biology Chemistry Mucin Mucins General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Molecular biology Healthy Volunteers In vitro Gastrointestinal Microbiome Intestines 030104 developmental biology 0210 nano-technology Bacteria Protein Binding |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 165:235-238 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | Cells of E. coli isolates from the gut of healthy volunteers (N=5) and patients with Crohn's disease (N=5) and laboratory E. coli strain DH5α bound mucin in vitro in similar amounts ranging from 0.02 to 0.12 mg/mg of bacterial dry weight. Binding was evaluated by the decrease in optical absorption of mucin solution at 214 nm after incubation with bacteria. Detailed analysis of mucin binding by one of isolates showed that during incubation of 0.09 mg/ml bacteria in 0.15 M NaCl containing 0.1 mg/ml mucin at 25oC, maximum binding was reached in 30 min, while in the presence of 14 mM α-methyl mannoside, mucin binding decreased by 46% (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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