Efficient Neural Differentiation of hPSCs by Extrinsic Signals Derived from Co-cultured Neural Stem or Precursor Cells
| Autor: | Sang Hun Lee, Yong Hee Rhee, Jae-Won Shim, Lesly Puspita, Seung Won Kim, Mi Yoon Chang, Yanuar Alan Sulistio, Rosalie Elvira, Vincencius Vidyawan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Programmed cell death
Green Fluorescent Proteins Induced Pluripotent Stem Cells Cell Culture Techniques Biology 03 medical and health sciences 0302 clinical medicine Neural Stem Cells Precursor cell Cell Line Tumor Drug Discovery Genetics Humans Induced pluripotent stem cell Molecular Biology Embryonic Stem Cells 030304 developmental biology Pharmacology 0303 health sciences Cell Differentiation Coculture Techniques Cell biology Phenotype 030220 oncology & carcinogenesis Molecular Medicine Neural differentiation Original Article Fibroblast Growth Factor 2 Nervous System Diseases Octamer Transcription Factor-3 Stem Cell Transplantation |
| Zdroj: | Mol Ther |
| Popis: | In this study, we found that undifferentiated human pluripotent stem cells (hPSCs; up to 30% of total cells) present in the cultures of neural stem or precursor cells (NPCs) completely disappeared within several days when cultured under neural differentiation culture conditions. Intriguingly, the disappearance of undifferentiated cells was not due to cell death but was instead mediated by neural conversion of hPSCs. Based on these findings, we propose pre-conditioning of donor NPC cultures under terminal differentiation culture conditions as a simple but efficient method of eliminating undifferentiated cells to treat neurologic disorders. In addition, we could establish a new neural differentiation protocol, in which undifferentiated hPSCs co-cultured with NPCs become differentiated neurons or NPCs in an extremely efficient, fast, and reproducible manner across the hESC and human-induced pluripotent stem cell (hiPSC) lines. |
| Databáze: | OpenAIRE |
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