Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

Autor: Hafiz M.N. Iqbal, Jayanthi Narayanan, K.M. Aguilar-Pérez, Roberto Parra-Saldívar, Dora I. Medina
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Antifungal Agents
oregano essential oil
Static Electricity
Dispersity
Pharmaceutical Science
Organic chemistry
02 engineering and technology
Article
Analytical Chemistry
law.invention
03 medical and health sciences
QD241-441
Dynamic light scattering
law
Origanum
Spectroscopy
Fourier Transform Infrared
Drug Discovery
Oils
Volatile
Zeta potential
particle size distribution
Particle Size
Physical and Theoretical Chemistry
Unilamellar Liposomes
Essential oil
030304 developmental biology
0303 health sciences
Mycelium
biology
Chemistry
Vesicle
Bilayer
antifungal activity
Fungi
021001 nanoscience & nanotechnology
biology.organism_classification
mycelial growth inhibition
Chemistry (miscellaneous)
Attenuated total reflection
Phosphatidylcholines
Nanoparticles
Molecular Medicine
nanoliposomes
0210 nano-technology
Nuclear chemistry
Zdroj: Molecules, Vol 26, Iss 2880, p 2880 (2021)
Molecules
Volume 26
Issue 10
ISSN: 1420-3049
Popis: The development of greener nano-constructs with noteworthy biological activity is of supreme interest, as a robust choice to minimize the extensive use of synthetic drugs. Essential oils (EOs) and their constituents offer medicinal potentialities because of their extensive biological activity, including the inhibition of fungi species. However, their application as natural antifungal agents are limited due to their volatility, low stability, and restricted administration routes. Nanotechnology is receiving particular attention to overcome the drawbacks of EOs such as volatility, degradation, and high sensitivity to environmental/external factors. For the aforementioned reasons, nanoencapsulation of bioactive compounds, for instance, EOs, facilitates protection and controlled-release attributes. Nanoliposomes are bilayer vesicles, at nanoscale, composed of phospholipids, and can encapsulate hydrophilic and hydrophobic compounds. Considering the above critiques, herein, we report the in-house fabrication and nano-size characterization of bioactive oregano essential oil (Origanum vulgare L.) (OEO) molecules loaded with small unilamellar vesicles (SUV) nanoliposomes. The study was focused on three main points: (1) multi-compositional fabrication nanoliposomes using a thin film hydration–sonication method
(2) nano-size characterization using various analytical and imaging techniques
and (3) antifungal efficacy of as-developed OEO nanoliposomes against Trichophyton rubrum (T. rubrum) by performing the mycelial growth inhibition test (MGI). The mean size of the nanoliposomes was around 77.46 ± 0.66 nm and 110.4 ± 0.98 nm, polydispersity index (PdI) of 0.413 ± 0.015, zeta potential values up to −36.94 ± 0.36 mV were obtained by dynamic light scattering (DLS). and spherical morphology was confirmed by scanning electron microscopy (SEM). The presence of OEO into nanoliposomes was displayed by attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. Entrapment efficiency values of 79.55 ± 6.9% were achieved for OEO nanoliposomes. In vitro antifungal activity of nanoliposomes tested against T. rubrum strains revealed that OEO nanoliposomes exhibited the highest MGI, 81.66 ± 0.86%, at a concentration of 1.5 µL/mL compared to the rest of the formulations. In summary, this work showed that bioactive OEO molecules with loaded nanoliposomes could be used as natural antifungal agents for therapeutical purposes against T. rubrum.
Databáze: OpenAIRE
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