Supplementary Figure 3 from FOXC2 Expression Links Epithelial–Mesenchymal Transition and Stem Cell Properties in Breast Cancer

Autor: Sendurai A. Mani, Jeffrey M. Rosen, Naoyuki Miura, Jeffrey T. Chang, Rudy Guerra, Jason I. Herschkowitz, Venkata L. Battula, Sreedevi V. Kumar, Maryam Shariati, Nathalie Sphyris, Tapasree Roy Sarkar, Joseph H. Taube, Kurt W. Evans, Steven J. Werden, Agata A. Tinnirello, Brett G. Hollier
Rok vydání: 2023
Popis: Supplementary Figure 3 PDF File - 326K, Attenuation of FOXC2 expression leads to reduced levels of PDGFR- �. (a) Immunofluorescene staining for PDGFR-� (red) detected with Alexa Fluor 548 anti-mouse secondary antibody in HMLE, HMLE-Snail-shCntrl and HMLE-Snail-shFOXC2 cells. Nuclei were counterstained with DAPI and overlayed images are shown.Scale bar indicates 100 �m. (b) FACS analysis of PDGFR-� cell surface expression using anti- PDGFR-�-PE antibody in cells described in (a). The histogram represents the intensity of PE signal in the FL2 detector on the x-axis
Databáze: OpenAIRE