Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design
| Autor: | Zong-Ming Zheng, Dehua Liu, Yan Sun, Jian Hao, Qiu-long Hu, Ni-Ni Guo, Feng Xu |
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| Rok vydání: | 2006 |
| Předmět: |
Glycerol
Environmental Engineering Time Factors Central composite design Klebsiella pneumoniae Bioengineering Propanediol chemistry.chemical_compound Bioreactors 1 3-Propanediol Response surface methodology Waste Management and Disposal Bacteriological Techniques Chromatography biology Renewable Energy Sustainability and the Environment Chemistry business.industry Air General Medicine Hydrogen-Ion Concentration biology.organism_classification Biotechnology Culture Media Propylene Glycols Fermentation Aeration business |
| Zdroj: | Bioresource technology. 99(5) |
| ISSN: | 0960-8524 |
| Popis: | A central composite design was used to study the effect of glycerol, rate of stirring, air aeration and pH on the synthesis of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae AC 15. Among the four variables, glycerol and rate of stirring significantly affected 1,3-PD productivity, whereas air aeration and pH were not effective. A quadratic polynomial equation was obtained for 1,3-PD productivity by multiple regression analysis using response surface methodology. The validation experimental confirmed with the predicted model. The optimum combinations for 1,3-PD productivity was glycerol, rate of stirring, air aeration, and pH of 50 g/l, 318 rpm, 0.6 vvm, 6.48, respectively. The subsequent fed batch experiments produced 1,3-PD of 70 g/l at a fermentation of 30 h. |
| Databáze: | OpenAIRE |
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