Evaluation of Microscopic Observation Drug Susceptibility (MODS) and the string test for rapid diagnosis of pulmonary tuberculosis in HIV/AIDS patients in Bolivia

Autor: Magaly Espinoza, Maya Vallejo, Roxana Challapa, Rosario Castro, Faustino Torrico, Robert H. Gilman, Melissa J. Reimer-McAtee, Meredith Holtz Lora, Marco Solano, Monica J. Pajuelo, Caryn Bern, Ruth Saravia, Daniel Lozano
Rok vydání: 2015
Předmět:
Male
Pathology
Opportunistic infection
Antitubercular Agents
Sputum/microbiology
MODS
Gastroenterology
fluids and secretions
Medical microbiology
Tuberculosis
Multidrug-Resistant
Cause of death
Microscopy
medicine.diagnostic_test
biology
Acquired Immunodeficiency Syndrome/complications/diagnosis
Middle Aged
3. Good health
AIDS
Infectious Diseases
Female
Tuberculosis
Pulmonary/complications/diagnosis/microbiology
medicine.symptom
Microscopy/methods
Research Article
Resource-scarce
Adult
Bolivia
medicine.medical_specialty
Tuberculosis
Multidrug-Resistant/complications/diagnosis/microbiology
Tuberculosis
Urinalysis
Microbial Sensitivity Tests
purl.org/pe-repo/ocde/ford#3.03.08 [https]
Sensitivity and Specificity
Mycobacterium tuberculosis
Acquired immunodeficiency syndrome (AIDS)
Internal medicine
medicine
Humans
String test
Tuberculosis
Pulmonary
Antitubercular Agents/pharmacology
Acquired Immunodeficiency Syndrome
business.industry
Sputum
HIV
medicine.disease
biology.organism_classification
Cross-Sectional Studies
Mycobacterium tuberculosis/drug effects/isolation & purification
Microbial Sensitivity Tests/methods
business
Zdroj: BMC Infectious Diseases
ISSN: 1471-2334
DOI: 10.1186/s12879-015-0966-0
Popis: Background: Tuberculosis (TB) is the most common opportunistic infection and the leading cause of death in HIV-positive people worldwide. Diagnosing TB is difficult, and is more challenging in resource-scarce settings where culture-based diagnostic methods rely on poorly sensitive smear microscopy by Ziehl-Neelsen stain (ZN). Methods: We performed a cross-sectional study examining the diagnostic utility of Microscopic Observation Drug Susceptibility liquid culture (MODS) versus traditional Ziehl-Neelsen staining (ZN) and Lowenstein Jensen culture (LJ) of pulmonary tuberculosis (TB) and multidrug-resistant tuberculosis (MDRTB) in HIV-infected patients in Bolivia. For sputum scarce individuals we assessed the value of the string test and induced sputum for TB diagnosis. The presence of Mycobacterium tuberculosis (Mtb) in the sputum of 107 HIV-positive patients was evaluated by ZN, LJ, and MODS. Gastric secretion samples obtained by the string test were evaluated by MODS in 102 patients. Results: The TB-HIV co-infection rate of HIV patients with respiratory symptoms by sputum sample was 45 % (48/107); 46/48 (96 %) were positive by MODS, 38/48 (79 %) by LJ, and 30/48 (63 %) by ZN. The rate of MDRTB was 9 % (4/48). Median time to positive culture was 10 days by MODS versus 34 days by LJ (p
Databáze: OpenAIRE
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