Evidence for existence of polyol pathway in cultured rat mesangial cells
| Autor: | Kazuhiko Ebata, Masakazu Haneda, Kisaburo Umemura, Yukio Shigeta, Ryuichi Kikkawa, Tetsuro Arimura |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
L-Iditol 2-Dehydrogenase Sorbitol dehydrogenase Endocrinology Diabetes and Metabolism Biology Diabetes Mellitus Experimental chemistry.chemical_compound Polyol pathway Sugar Alcohols Polyol Aldehyde Reductase Internal Medicine medicine Animals Sorbitol Cells Cultured chemistry.chemical_classification Aldose reductase Mesangial cell Rats Inbred Strains Aldose reductase inhibitor Glomerular Mesangium Rats chemistry Biochemistry Cell culture Galactitol medicine.drug |
| Zdroj: | Diabetes. 36(2) |
| ISSN: | 0012-1797 |
| Popis: | The accumulation of polyols has been previously found in renal glomeruli isolated from streptozocin-induced diabetic (STZ-D) rats, although the intraglomerular polyol pathway has not been exactly localized. Because we have previously observed mesangial cell dysfunction in STZ-D rats, we examined whether the polyol pathway exists in mesangial cells as a possible candidate of the cause of cellular dysfunction. The activities of two polyol pathway enzymes, aldose reductase and sorbitol dehydrogenase, were clearly detected in the crude homogenate of cultured mesangial cells at higher levels than those of whole glomeruli when DL-glyceraldehyde or D-fructose was used as substrate. When cells were incubated in medium containing 55 mM glucose or galactose, a large amount of sorbitol or galactitol was accumulated intracellularly. The accumulation of polyolswas effectively blocked by an aldose reductase inhibitor, ICI 128436. These results suggest that the polyol pathway exists in mesangial cells of rat glomeruli and may play a role in the development of mesangial cell dysfunction found in STZ-D rats. |
| Databáze: | OpenAIRE |
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