Molecular and genetic organization of Drosophila melanogasterpolytene chromosomes: evidence for two types of interband regions
| Autor: | S. A. Demakov, Sonsoles Campuzano, Andrei Gortchakov, V. F. Semeshin, Juan Modolell, Igor F. Zhimulev, Yuri B. Schwartz |
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| Rok vydání: | 2004 |
| Předmět: |
clone (Java method)
Transposable element Plant Science Chromosomes chemistry.chemical_compound Genetics Animals Cloning Molecular Drosophila (subgenus) Gene Polytene chromosome biology Genetic heterogeneity Sequence Analysis DNA General Medicine biology.organism_classification Chromosome Banding Microscopy Electron Drosophila melanogaster chemistry Insect Science DNA Transposable Elements Animal Science and Zoology DNA |
| Zdroj: | Genetica. 122:311-324 |
| ISSN: | 1573-6857 0016-6707 |
| Popis: | The 3A and 60E regions of Drosophila melanogaster polytene chromosomes containing inserted copies of the P[1ArB] transposon have been subjected to an electron microscopic (EM) analysis. We show that both inserts led to formation of new bands within the interband regions 3A4/A6 and 60E8-9/E10. This allowed us to clone DNA of these interbands. Their sequences, as well as those of DNA from other four interbands described earlier, have been analyzed. We have found that, with the exception of 60E8-9/E10 interband, all other five regions under study corresponded to 5' or 3' ends of genes. We have further obtained the evidence for 60E8-9/E10 interband to harbor the 'housekeeping' RpL19 gene, which is transcribed in many tissues, including salivary glands. Based upon the genetic heterogeneity of the interbands observed a revised model of polytene chromosome organization is discussed. |
| Databáze: | OpenAIRE |
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