An armed oncolytic adenovirus system, ZD55-gene, demonstrating potent antitumoral efficacy
| Autor: | Bing Hua Li, Weiguo Zou, Jinhui Wang, Lan Ying Sun, Xinyuan Liu, Chun Xia Luo, Qi Jun Qian, Zi Lai Zhang |
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| Rok vydání: | 2004 |
| Předmět: |
Oncolytic adenovirus
Carcinoma Hepatocellular Genetic Vectors Transplantation Heterologous Mice Nude Antineoplastic Agents Biology Virus Replication Virus Adenoviridae Mice Cytopathogenic Effect Viral In vivo Genes Reporter Cell Line Tumor medicine Animals Humans Molecular Biology Cells Cultured Regulation of gene expression Mice Inbred BALB C Cancer Cell Biology Genetic Therapy Oncogenes medicine.disease Virology Transplantation Gene Expression Regulation Neoplastic Viral replication Cell culture Female Fluorouracil Colorectal Neoplasms Neoplasm Transplantation HeLa Cells |
| Zdroj: | Cell research. 13(6) |
| ISSN: | 1001-0602 |
| Popis: | ONYX-015 is an attractive therapeutic adenovirus for cancer because it can selectively replicate in tumor cells and kill them. To date, clinical trials of this adenovirus have demonstrated marked safety but not potent enough when it was used alone. In this paper, we put forward a novel concept of Gene-ViroTherapy strategy and in this way, we constructed an armed therapeutic oncolytic adenovirus system, ZD55-gene, which is not only deleted of E1B 55-kD gene similar to ONYX-015, but also armed with foreign antitumor gene. ZD55-gene exhibited similar cytopathic effects and replication kinetics to that of ONYX-015 in vitro. Importantly, the carried gene is expressed and the expression level can increase with the replication of virus. Consequently, a significant antitumoral efficacy was observed when ZD55-CD/5-FU was used as an example in nude mice with subcutaneous human SW620 colon cancer. Our data demonstrated that ZD55-gene, which utilizing the Gene-ViroTherapy strategy, is more efficacious than each individual component in vivo. |
| Databáze: | OpenAIRE |
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