The effect of thawing velocity on survival and acrosomal integrity of ram spermatozoa frozen at optimal and suboptimal rates in straws
| Autor: | R.W. Fairfull, G.J. Marcus, P.S. Fiser |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
endocrine system Cell Survival medicine.medical_treatment Motility Semen Biology General Biochemistry Genetics and Molecular Biology Cryopreservation Andrology chemistry.chemical_compound Freezing Glycerol medicine Animals Acrosome Sheep urogenital system Artificial insemination General Medicine Spermatozoa Kinetics chemistry General Agricultural and Biological Sciences Mathematics |
| Zdroj: | Cryobiology. 23:141-149 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/0011-2240(86)90005-2 |
| Popis: | The effect of various thawing velocities on the motility and acrosomal maintenance of ram spermatozoa frozen at 20 degrees C/min (optimal) or 2 degrees C/min (suboptimal) was studied. The freeze-thaw motility and the percentage of intact acrosomes of spermatozoa frozen at 20 degrees C/min increased progressively with the thawing velocity. In semen frozen at 2 degrees C/min, motility of spermatozoa and the percentage of intact acrosomes declined drastically when the thawing velocity obtained in air at 20 degrees C was increased by thawing in water at 20 degrees C. Thawing at higher temperatures markedly increased both motility and acrosomal preservation, but the best results with semen frozen at 2 degrees C/min were lower than those obtained with semen frozen at 20 degrees C/min. The optimal freeze-thaw conditions for semen protected by 4% glycerol were freezing at 20 degrees C/min and thawing in water at 60 or 80 degrees C for 8 or 5 sec, respectively. Semen collected from rams exposed to a decreasing photoperiod exhibited higher motility after freezing and thawing than those exposed to an increasing photoperiod. However, there was no effect on acrosomal preservation after freezing at 20 degrees C/min. |
| Databáze: | OpenAIRE |
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