The role of upregulated miRNAs and the identification of novel mRNA targets in prostatospheres
| Autor: | Elaine M. Hurt, James Cherry, Stephanie M. Cabarcas, Eric Lader, Xiaohu Zhang, Thomas Sebastian, William L. Farrar, Subu Yerramilli, Suneetha B. Thomas |
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| Rok vydání: | 2012 |
| Předmět: |
Male
congenital hereditary and neonatal diseases and abnormalities Biology Real-Time Polymerase Chain Reaction Genome Tumor-initiating cell Article Cancer stem cell Spheroids Cellular microRNA Gene expression mental disorders Tumor Cells Cultured Genetics Gene silencing Humans RNA Messenger Gene Prostatospheres Cell Proliferation Oligonucleotide Array Sequence Analysis Regulation of gene expression Prostate cancer Cancer stem cells Gene Expression Profiling Prostatic Neoplasms Cell biology nervous system diseases Up-Regulation Gene expression profiling body regions Gene Expression Regulation Neoplastic MicroRNAs Neoplastic Stem Cells Neoplasm Recurrence Local human activities |
| Zdroj: | Genomics. 99(2):108-117 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2011.11.007 |
| Popis: | TICs are characterized by their ability to self-renew, differentiate and initiate tumor formation. miRNAs are small noncoding RNAs that bind to mRNAs resulting in regulation of gene expression and biological functions. The role of miRNAs and TICs in cancer progression led us to hypothesize that miRNAs may regulate genes involved in TIC maintenance. Using whole genome miRNA and mRNA expression profiling of TICs from primary prostate cancer cells, we identified a set of up-regulated miRNAs and a set of genes down-regulated in PSs. Inhibition of these miRNAs results in a decrease of prostatosphere formation and an increase in target gene expression. This study uses genome-wide miRNA profiling to analyze expression in TICs. We connect aberrant miRNA expression and deregulated gene expression in TICs. These findings can contribute to a better understanding of the molecular mechanisms governing TIC development/maintenance and the role that miRNAs have in the fundamental biology of TICs. |
| Databáze: | OpenAIRE |
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