Effect of a bacteriophage cocktail in combination with modified atmosphere packaging in controlling listeria monocytogenes on fresh-cut spinach
| Autor: | Olcay Boyacioglu, Manan Sharma, Alexander Sulakvelidze, Ipek Goktepe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
L. monocytogenes spinach 030106 microbiology Modified atmosphere packaging medicine.disease_cause Bacteriophage 03 medical and health sciences Plant science Listeria monocytogenes bacteriophage medicine Food science biocontrol lcsh:Agriculture (General) modified atmosphere packaging 030102 biochemistry & molecular biology Ecology biology business.industry Biocontrol Spinach biology.organism_classification lcsh:S1-972 Agriculture Modified atmosphere Animal Science and Zoology business Agronomy and Crop Science L monocytogenes Food Science |
| Zdroj: | Irish Journal of Agricultural and Food Research, Vol 55, Iss 1, Pp 74-79 (2016) |
| Popis: | AListeria monocytogenes-specific bacteriophage cocktail was evaluated for its activity against a nalidixic acid-resistantL. monocytogenes(Lm-NalR) isolate on fresh-cut spinach stored under modified atmosphere packaging at various temperatures. Pieces (~2 × 2 cm2) of fresh spinach inoculated with 4.5 log CFU/cm2Lm-NalRwere sprayed with the phage cocktail (6.5 log plaque-forming units [PFU]/cm2) or a control. The samples were stored at 4°C or 10°C for up to 14 d in sealed packages filled with either atmospheric air (AA) or modified atmosphere (MA). At 4°C under AA, the phages significantly (P≤ 0.05) lowered theLm-NalRpopulations on spinach, compared to control-treated inoculated samples, by 1.12 and 1.51 log CFU/cm2after 1 and 14 d, respectively. At 4°C under MA,Lm-NalRwas significantly reduced by 1.95 log CFU/cm2compared to control leaves after both 1 and 14 d. At 10°C under AA, the phages significantly reducedLm-NalRby 1.50 and 2.51 log CFU/cm2after 1 and 14 d compared to the control. Again at 10°C under MA, the phages significantly reducedLm-NalRby 1.71 and 3.24 log CFU/cm2compared to control after 1 and 14 d, respectively. The results support the potential of lytic bacteriophages in effectively reducing populations ofL. monocytogeneson freshcut leafy produce, under both AA and MA conditions. |
| Databáze: | OpenAIRE |
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