Anti-Melanogenic Potentials of Nanoparticles from Calli of Resveratrol-Enriched Rice against UVB-Induced Hyperpigmentation in Guinea Pig Skin
| Autor: | So-Hyeon Baek, Jae Ok Seo, Taek Hwan Lee, Sun Yeou Kim, Young Ko, Yong Un Park, Ji Hee Kang, Sang Hyun Moh, Jae Kyoung Chae |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Melanogenesis Tyrosinase Plant tissue culture Resveratrol Biology Biochemistry Guinea pig Melanin 03 medical and health sciences chemistry.chemical_compound Depigmentation Drug Discovery Botany medicine Pharmacology integumentary system High-pressure homogenization Hyperpigmentation Molecular biology 030104 developmental biology Resveratrol-enriched rice chemistry Molecular Medicine Nanoparticles Original Article medicine.symptom Immunostaining |
| Zdroj: | Biomolecules & Therapeutics |
| ISSN: | 1976-9148 |
| Popis: | We already reported that genetically engineered resveratrol-enriched rice (RR) showed to down-regulate skin melanogenesis. To be developed to increase the bioactivity of RR using calli from plants, RR was adopted for mass production using plant tissue culture technologies. In addition, high-pressure homogenization (HPH) was used to increase the biocompatibility and penetration of the calli from RR into the skin. We aimed to develop anti-melanogenic agents incorporating calli of RR (cRR) and nanoparticles by high-pressure homogenization, examining the synergistic effects on the inhibition of UVB-induced hyperpigmentation. Depigmentation was observed following topical application of micro-cRR, nano-calli of normal rice (cNR), and nano-cRR to ultraviolet B (UVB)-stimulated hyperpigmented guinea pig dorsal skin. Colorimetric analysis, tyrosinase immunostaining, and Fontana-Masson staining for UVB-promoted melanin were performed. Nano-cRR inhibited changes in the melanin color index caused by UVB-promoted hyperpigmentation, and demonstrated stronger anti-melanogenic potential than micro-cRR. In epidermal skin, nano-cRR repressed UVB-promoted melanin granules, thereby suppressing hyperpigmentation. The UVB-enhanced, highly expressed tyrosinase in the basal layer of the epidermis was inhibited by nano-cRR more prominently than by micro-cRR and nano-cNR. The anti-melanogenic potency of nano-cRR also depended on pH and particle size. Nano-cRR shows promising potential to regulate skin pigmentation following UVB exposure. |
| Databáze: | OpenAIRE |
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