Characterization, cerebral distribution and gonadotropin release activity of neuropeptide Y (NPY) in the goldfish
| Autor: | A. Calas, Olivier Kah, Hubert Vaudry, P. Dubourg, A. Pontet, G. Pelletier, J. M. Danger |
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| Rok vydání: | 2013 |
| Předmět: |
endocrine system
medicine.medical_specialty Pituitary gland Physiology medicine.drug_class Cerebrum Immunocytochemistry Central nervous system Radioimmunoassay General Medicine Aquatic Science Biology Neuropeptide Y receptor Biochemistry humanities Diencephalon Endocrinology medicine.anatomical_structure Internal medicine mental disorders medicine Gonadotropin |
| Zdroj: | Fish physiology and biochemistry. 7(1-6) |
| ISSN: | 0920-1742 |
| Popis: | The presence of a peptide closely related to porcine NPY has been demonstrated in the goldfish brain and pituitary by means of radioimmunoassay (RIA) and high performance liquid chromatography (HPLC). The RIA data demonstrate that displacement curves of brain extracts are parallel to a porcine NPY standard and that in HPLC a compound present in brain extracts is co-eluted with porcine NPY. The distribution of this NPY-like factor within the central nervous system was studied by radioimmunoassay and immunohistochemistry. The results indicated that NPY has a widespread distribution with the highest concentrations being found in the telencephalon and diencephalon. In the pituitary gland, NPY immunoreactive terminals characterized at the electron microscope level were found in the different lobes and, in particular, in close association with the gonadotrophin (GTH) secreting cells. Using anin vitro perifusion system, it was shown that NPY causes a dose dependent increase of GTH release from anterior lobe fragments.These data indicate for the first time in teleosts that NPY is present and widely distributed in the brain and pituitary, and that among other putative functions, could be implicated in the multihormonal release of GTH from the pituitary. |
| Databáze: | OpenAIRE |
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