Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome
| Autor: | Simona Menšíková, Miroslav Berka, Aaron M. Rashotte, Markéta Luklová, Romana Kopecká, Břetislav Brzobohatý, Jan Novák, Michaela Kameniarová, Lukáš Spíchal, Vladěna Ondrisková, Veronika Berková, Martin Černý |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis thaliana proteome Plant Science 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound cytokinin Arabidopsis lcsh:Botany Cytokinin dehydrogenase Secondary metabolism Abscisic acid Ecology Evolution Behavior and Systematics Ecology biology Phenylpropanoid CKX fungi food and beverages biology.organism_classification Cell biology lcsh:QK1-989 030104 developmental biology inhibitor of cytokinin degradation chemistry Cytokinin Proteome stress response attenuation 010606 plant biology & botany |
| Zdroj: | Plants, Vol 9, Iss 1563, p 1563 (2020) Plants Volume 9 Issue 11 |
| ISSN: | 2223-7747 |
| Popis: | Cytokinins are multifaceted plant hormones that play crucial roles in plant interactions with the environment. Modulations in cytokinin metabolism and signaling have been successfully used for elevating plant tolerance to biotic and abiotic stressors. Here, we analyzed Arabidopsis thaliana response to INhibitor of CYtokinin DEgradation (INCYDE), a potent inhibitor of cytokinin dehydrogenase. We found that at low nanomolar concentration, the effect of INCYCE on seedling growth and development was not significantly different from that of trans-Zeatin treatment. However, an alteration in the spatial distribution of cytokinin signaling was found at low micromolar concentrations, and proteomics analysis revealed a significant impact on the molecular level. An in-depth proteome analysis of an early (24 h) response and a dose-dependent response after 168 h highlighted the effects on primary and secondary metabolism, including alterations in ribosomal subunits, RNA metabolism, modulations of proteins associated with chromatin, and the flavonoid and phenylpropanoid biosynthetic pathway. The observed attenuation in stress-response mechanisms, including abscisic acid signaling and the metabolism of jasmonates, could explain previously reported positive effects of INCYDE under mild stress conditions. |
| Databáze: | OpenAIRE |
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