Young Adult Chondrocytes Proliferate Rapidly and Produce a Cartilaginous Tissue at the Gel-Media Interface in Agarose Cultures

Autor:	Michael D. Buschmann, Nicolas Tran-Khanh, Anik Chevrier, Caroline D. Hoemann, V. Lascau-Coman
Rok vydání:	2010
Předmět:	Cartilage Articular Male Aging Cell division Cell Count Cell morphology Biochemistry Chondrocyte Extracellular matrix Chondrocytes Fetus Rheumatology Tissue engineering Cell Movement Cartilaginous Tissue medicine Animals Orthopedics and Sports Medicine Molecular Biology Endochondral ossification Cells Cultured Cytoskeleton Cell Proliferation Tissue Engineering Chemistry Sepharose Cartilage DNA Cell Biology Anatomy Extracellular Matrix Cell biology Ki-67 Antigen medicine.anatomical_structure Cattle Gels
Zdroj:	Connective Tissue Research. 51:216-223
ISSN:	1607-8438 0300-8207
DOI:	10.3109/03008200903281683
Popis:	Primary chondrocytes cultured in agarose can escape the gel, accumulate at the interface between agarose and the culture medium, and form an outgrowing tissue. These outgrowths can appear as voluminous cartilage-like nodules that have never been previously investigated. In the present study, bovine articular chondrocytes from three age groups (fetal, young adult, aged) were seeded and cultured in agarose to test the hypothesis that hyaline-like cartilage outgrowths develop at the interface by appositional growth, in an age-dependant manner. Macroscopic appearance, cell content, cell division, cytoskeletal morphology, and extracellular matrix (ECM) composition were analyzed. Fetal chondrocytes produced a fibrous interfacial tissue while aged chondrocytes produced ECM-poor cell clusters. In contrast young adult chondrocytes produced large cartilaginous outgrowths, rich in proteoglycan and collagen II, where cells in the central region displayed a chondrocyte morphology. Cell proliferation was confined to the peripheral edge of these outgrowths, where elongated cell morphology, cell-cell contacts, and cell extensions toward the culture medium were seen. Thus these voluminous cartilaginous outgrowths formed in an appositional growth process and only for donor chondrocytes from young adult animals. This system offers an interesting ability to proliferate chondrocytes in a manner that results in a chondrocyte morphology and a cartilaginous ECM in central regions of the outgrowing tissue. It also provides an in vitro model system to study neocartilage appositional growth.
Databáze:	OpenAIRE
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