Chemical Characterization and Wound Healing Property of Jacaranda decurrens Cham. (Bignoniaceae): An Experimental Study Based on Molecular Mechanisms

Autor: Denise Frediani Barbeiro, Wermerson Assunção Barroso, Antonio Carlos Romão Borges, Heraldo Possolo de Souza, Selma do Nascimento Silva, Marilene Oliveira da Rocha Borges, Pablo G.R. Furtado, Iracelle Carvalho Abreu, Ademilton Costa Alves, Wagner Vilegas, Mariana Barreto Serra, Jessyane R. do Nascimento, Marcelo Marucci Pereira Tangerina, Cláudia Quintino da Rocha
Přispěvatelé: Federal University of Maranhaõ, University of Saõ Paulo, Rio Branco, Universidade Estadual Paulista (Unesp)
Rok vydání: 2020
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine, Vol 2020 (2020)
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1741-4288
1741-427X
DOI: 10.1155/2020/4749712
Popis: Made available in DSpace on 2020-12-12T02:40:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Background. Jacaranda decurrens Cham., known as carobinha, is prevalent in the Cerrado biome and presents popular use in treatment of dermatological diseases. The present study aimed to investigate the healing action of topical formulation of Jacaranda decurrens Cham. (FtEHJ) in mice cutaneous lesions. Methods. Phytochemical analysis of J. decurrens hydroalcoholic extract was carried out by using HPLC-PDA-ESI-MS and FIA-ESI-IT-MSn. Swiss mice were treated topically with formulation base (FtB) or Fibrinase® or ointment FtEHJ (15 mg/g; 50 mg/Kg). At the end of treatment periods, the inflammatory cytokines (TNF-, IL-1β, and IL-6) in the lesions were measured by using ELISA and gene expression of TGF-β, Collagen I, and Collagen III was demonstrated by RTqPCR method and histological evaluation. Results. Ten compounds were identified in the extract, distributed among the classes of flavonoids and triterpenes. Treatment with FtEHJ increased the wound contraction in 24 hours, such as reduction of TNF-, IL-1β, and IL-6 (pg/mL) cytokines in the lesion. The TGF-β and collagen gene expression was increased and the wound closure accelerated to nine days, with discrete inflammation, collagenization, and accented reepithelialization. Conclusions. The results obtained suggest chemical compounds present in the FtEHJ accelerates wound healing by being a gene expression modulator, and protein content of different molecules are involved in tissue repair. Physiological Sciences Department Federal University of Maranhaõ Clinical Medicine Department University of Saõ Paulo Department of Chemistry Federal University of Maranhaõ DNA Medical Diagnóstics Rio Branco Institute of Biosciences Paulista State University (UNESP) Pharmacy Department Federal University of Maranhaõ Institute of Biosciences Paulista State University (UNESP)
Databáze: OpenAIRE
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