Effect of isoflavone extracts from Glycine max on human endothelial cell damage and on nitric oxide production
| Autor: | Mário dos Anjos Neto Filho, Maria Regina Torqueti Toloi, Mirian Mendonça Salvador, Marlise Bonetti Agostinho Montes, Michele Paulo, Silvio Antonio Franceschini |
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| Rok vydání: | 2009 |
| Předmět: |
Umbilical Veins
Antioxidant Endothelium Cell Survival medicine.medical_treatment Nitric Oxide Antioxidants Nitric oxide Cell Line chemistry.chemical_compound medicine Estrogen Receptor beta Humans Cell damage business.industry Estrogen Receptor alpha Obstetrics and Gynecology Endothelial Cells Hydrogen Peroxide Isoflavones medicine.disease Genistein Endothelial stem cell Oxidative Stress medicine.anatomical_structure chemistry Biochemistry Phytoestrogens Human umbilical vein endothelial cell Soybeans business |
| Zdroj: | Menopause (New York, N.Y.). 16(3) |
| ISSN: | 1530-0374 |
| Popis: | Objective: In this study, we determined the protective effect of isoflavones from Glycine max on human umbilical vein endothelial cell (ECV304) damage induced by hydrogen peroxide (H 2 O 2 ) and on nitric oxide (NO) production. Methods: We studied the regulation of NO synthesis in cultured human endothelial cells by phytoestrogens contained in soy extracts in the presence or absence of ICI 182,780 or N ω -nitro-L-arginine methyl esther and determined the protective effect of these isoflavones on ECV304 damage induced by H 2 O 2 . Results: We show that soy extracts activate NO synthesis in endothelial cells and protect against cell damage. Conclusions: In conclusion, soy isoflavones markedly protect ECV304 cells against H 2 O 2 damage and promote NO synthesizing. Therefore, these isoflavones can potentially act as an NO promoter and as an antioxidant. |
| Databáze: | OpenAIRE |
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