Cell Properties of Lung Tissue-Resident Macrophages Propagated by Co-Culture with Lung Fibroblastic Cells from C57BL/6 and BALB/c Mice
| Autor: | Haruka Horie, Kazushige Ogawa, Mayu Tsurutani |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
C57BL/6
lung interstitial macrophages QH301-705.5 Cell Population Medicine (miscellaneous) Article General Biochemistry Genetics and Molecular Biology BALB/c BALB/c mice medicine Biology (General) education C57BL/6 mice education.field_of_study Lung biology Chemistry Monocyte alveolar macrophages respiratory system MERTK biology.organism_classification co-culture Molecular biology respiratory tract diseases medicine.anatomical_structure Integrin alpha M biology.protein |
| Zdroj: | Biomedicines, Vol 9, Iss 1241, p 1241 (2021) Biomedicines Volume 9 Issue 9 |
| ISSN: | 2227-9059 |
| DOI: | 10.3390/biomedicines9091241 |
| Popis: | Tissue-resident macrophages (Mø) originating from foetal precursors are maintained by self-renewal under tissue/organ-specific microenvironments (niches). We recently developed a simple propagation method applicable to tissue-resident Mø by co-culturing. Here, we examined the properties of lung tissue-resident Mø propagated by co-culturing with lung interstitial cells. The intracardially and intratracheally perfused lung from BALB/c and C57BL/6 mice could minimise the contamination of alveolar Mø and lung monocytes. Lung tissue-resident Mø could be largely propagated under standard culture media along with the propagation of lung interstitial cells demonstrating a fibroblastic morphology. Propagated lung Mø showed characteristic expression properties for Mø/monocyte markers: high expressions of CD11b, CD64 and CD206 substantial expressions of Mertk and negative expressions of Ly6C, MHC II and Siglec-F. These properties fit with those of lung interstitial Mø of a certain population that can undergo self-renewal. Propagated fibroblastic cells by co-culturing with lung Mø possessed niche properties such as Csf1 and Tgfb1 expression. Propagated lung Mø from both the mouse types were polarised to an M2 phenotype highly expressing arginase 1 without M2 inducer treatment, whereas the M1 inducers significantly increased the iNOS-positive cell percentages in C57BL/6 mice relative to those in BALB/c mice. This is the first study to demonstrate fundamental properties of lung tissue-resident Mø propagated by co-culturing. Propagated lung Mø showing features of lung interstitial Mø can serve as an indispensable tool for investigating SARS-CoV-2 diseases, although lung interstitial Mø have gained little attention in terms of their involvement in SARS-CoV-2 disease pathology, in contrast to alveolar and recruited Mø. |
| Databáze: | OpenAIRE |
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