Reduction of calcium flux from the extracellular region and endoplasmic reticulum by amorphous nano-silica particles owing to carboxy group addition on their surface
| Autor: | Hideto Morosawa, Yuichi Kawai, Akira Onodera, Katsutoshi Yayama, Yukina Ishii, Yasuo Tsutsumi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
TRPV6 Calcium pump NM nanomaterial Biophysics chemistry.chemical_element 02 engineering and technology Balb/3T3 Balb/c 3T3 fibroblast Calcium Biochemistry ER endoplasmic reticulum lcsh:Biochemistry 03 medical and health sciences Calcium flux Calcium homeostasis Surface properties SP amorphous silica particle lcsh:QD415-436 lcsh:QH301-705.5 Calcium metabolism nSP70 nano-silica particle with a diameter of 70 nm Voltage-dependent calcium channel Endoplasmic reticulum 021001 nanoscience & nanotechnology Nanomaterial mSP1000 micro-silica particle with a diameter of 1000 nm Calcium ATPase 030104 developmental biology chemistry lcsh:Biology (General) VDCC voltage-dependent calcium channel nano-SP amorphous nano-silica particle nSP300 nano-silica particle with a diameter of 300 nm 0210 nano-technology Research Article |
| Zdroj: | Biochemistry and Biophysics Reports, Vol 9, Iss C, Pp 330-334 (2017) Biochemistry and Biophysics Reports |
| ISSN: | 2405-5808 |
| Popis: | Several studies have reported that amorphous nano-silica particles (nano-SPs) modulate calcium flux, although the mechanism remains incompletely understood. We thus analyzed the relationship between calcium flux and particle surface properties and determined the calcium flux route. Treatment of Balb/c 3T3 fibroblasts with nano-SPs with a diameter of 70 nm (nSP70) increased cytosolic calcium concentration, but that with SPs with a diameter of 300 or 1000 nm did not. Surface modification of nSP70 with a carboxy group also did not modulate calcium flux. Pretreatment with a general calcium entry blocker almost completely suppressed calcium flux by nSP70. Preconditioning by emptying the endoplasmic reticulum (ER) calcium stores slightly suppressed calcium flux by nSP70. These results indicate that nSP70 mainly modulates calcium flux across plasma membrane calcium channels, with subsequent activation of the ER calcium pump, and that the potential of calcium flux by nano-SPs is determined by the particle surface charge. Highlights • Nano-silica particles increased cytosolic calcium flux in fibroblasts. • Calcium flux by nano-SPs was suppressed by SKF96365 and thapsigargin. • Calcium flux modulation by nano-SPs was determined by their surface structure. |
| Databáze: | OpenAIRE |
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