Long-term in vitro persistence of magnetic properties after magnetic bead-based cell separation of T cells
| Autor: | Conny Hoogstraten, Aicha Laghmouchi, J. H. Frederik Falkenburg, I. Jedema |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
magnetic nanoparticles Cell division T-Lymphocytes Immunology Cell Cell Culture Techniques T cells Nanoparticle Lymphocyte Activation 03 medical and health sciences 0302 clinical medicine Cell separation medicine Humans Cell Proliferation Immunological Methods Immunomagnetic Separation Chemistry General Medicine Cell sorting Flow Cytometry equipment and supplies In vitro 030104 developmental biology medicine.anatomical_structure Magnetic bead Biophysics Magnetic nanoparticles MACS isolation Immunologic Memory human activities Biomarkers 030215 immunology |
| Zdroj: | Scandinavian Journal of Immunology, 92(3). WILEY Scandinavian Journal of Immunology |
| Popis: | Magnetic‐activated cell sorting (MACS) using magnetic nanoparticles coated with specific antibodies is commonly used in immunology research. For in vitro isolation purposes, it is important to know to what extent the magnetic properties remain present in the isolated cell populations and whether it has consequences for sequential isolations. We hypothesized that only upon cell division, cells will lose their magnetic properties via dilution of the particles in/on their daughter cells. We analysed residual magnetic properties of cells that divided vs cells that did not divide after magnetic bead‐based cell separation. As a model, we isolated T cells using beads targeting the non‐modulating surface molecule CD45RO. Cells were labelled with the cell division tracking dye PKH and cultured under different conditions to induce variable degrees of cell division. We demonstrate that T cells that underwent no, or only minimal, cell divisions after MACS retained magnetic properties for up to at least 2 weeks of in vitro culture. The presence of nanoparticles was detected on their cell surface and intracellularly using Labeling Check reagent. These results have important consequences for procedures requiring repetitive isolation rounds after in vitro culture. |
| Databáze: | OpenAIRE |
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