Mixed antagonist response and sepsis severity-dependent dysbalance of pro- and anti-inflammatory responses at the onset of postoperative sepsis
| Autor: | Bernhard Holzmann, Daniel Reim, Alexander Novotny, Helmut Friess, Felicitas Altmayr, Klaus Emmanuel, Volker Assfalg |
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| Rok vydání: | 2011 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_treatment Immunology Peritonitis Severity of Illness Index Cell Line Sepsis Mice Immune system Postoperative Complications medicine Immunology and Allergy Animals Humans Interleukin 6 Aged biology business.industry Interleukin-6 Macrophages Toll-Like Receptors Antagonist Hematology Middle Aged medicine.disease Systemic Inflammatory Response Syndrome Interleukin-10 Systemic inflammatory response syndrome Mice Inbred C57BL Interleukin 10 Cytokine biology.protein Female business Signal Transduction |
| Zdroj: | Immunobiology. 217(6) |
| ISSN: | 1878-3279 |
| Popis: | It has been postulated that an early systemic inflammatory response syndrome (SIRS) and a subsequent compensatory anti-inflammatory response syndrome (CARS) occur sequentially in sepsis. Co-existence of both is referred to as mixed antagonist response syndrome (MARS). Pro- and anti-inflammatory cytokine production was investigated in patients with postoperative sepsis, a murine peritonitis model and in vitro to further delineate the interaction of hyper- and hypo-inflammation in sepsis. IL-6 and IL-10 were measured in serum samples from 80 patients on d1 and d2 of postoperative sepsis and were similarly determined at various time points after induction of septic peritonitis in mice. Cytokine production of RAW264 macrophages was stimulated in vitro using TLR agonists. IL-6 and IL-10 were measured in supernatants. All cytokine measurements were performed by ELISA. In patients, the initial phase of the immune response to sepsis was characterized by a concomitant elevation of serum IL-6 and IL-10 levels. IL-10 levels were correlated with IL-6 levels in an exponential manner (p |
| Databáze: | OpenAIRE |
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