The Protein Corona around Nanoparticles Facilitates Stem Cell Labeling for Clinical MR Imaging
| Autor: | Seyed-Meghdad Taghavi-Garmestani, Saeid Zanganeh, Heike E. Daldrup-Link, Morteza Mahmoudi, Hossein Nejadnik, Phillip C. Yang, Kai Li, Steven J. Madsen |
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| Rok vydání: | 2018 |
| Předmět: |
endocrine system
Pathology medicine.medical_specialty Surface Properties Sus scrofa Cell Nanoparticle Protein Corona 02 engineering and technology Mesenchymal Stem Cell Transplantation Article 030218 nuclear medicine & medical imaging Cell labeling 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Scattering Radiation Radiology Nuclear Medicine and imaging In patient Particle Size Magnetite Nanoparticles business.industry Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Magnetic Resonance Imaging Mr imaging Ferrosoferric Oxide Culture Media Ferumoxytol medicine.anatomical_structure Cell Tracking Stem cell 0210 nano-technology business Chromatography Liquid Biomedical engineering |
| Zdroj: | Radiology. 286:938-947 |
| ISSN: | 1527-1315 0033-8419 |
| Popis: | Purpose To evaluate if the formation of a protein corona around ferumoxytol nanoparticles can facilitate stem cell labeling for in vivo tracking with magnetic resonance (MR) imaging. Materials and Methods Ferumoxytol was incubated in media containing human serum (group 1), fetal bovine serum (group 2), StemPro medium (group 3), protamine (group 4), and protamine plus heparin (group 5). Formation of a protein corona was characterized by means of dynamic light scattering, ζ potential, and liquid chromatography-mass spectrometry. Iron uptake was evaluated with 3,3'-diaminobenzidine-Prussian blue staining, lysosomal staining, and inductively coupled plasma spectrometry. To evaluate the effect of a protein corona on stem cell labeling, human mesenchymal stem cells (hMSCs) were labeled with the above formulations, implanted into pig knee specimens, and investigated with T2-weighted fast spin-echo and multiecho spin-echo sequences on a 3.0-T MR imaging unit. Data in different groups were compared by using a Kruskal-Wallis test. Results Compared with bare nanoparticles, all experimental groups showed significantly increased negative ζ values (from -37 to less than -10; P = .008). Nanoparticles in groups 1-3 showed an increased size because of the formation of a protein corona. hMSCs labeled with group 1-5 media showed significantly shortened T2 relaxation times compared with unlabeled control cells (P = .0012). hMSCs labeled with group 3 and 5 media had the highest iron uptake after cells labeled with group 1 medium. After implantation into pig knees, hMSCs labeled with group 1 medium showed significantly shorter T2 relaxation times than hMSCs labeled with group 2-5 media (P = .0022). Conclusion The protein corona around ferumoxytol nanoparticles can facilitate stem cell labeling for clinical cell tracking with MR imaging. |
| Databáze: | OpenAIRE |
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