Laminin-111-Enriched Fibrin Hydrogels Enhance Functional Muscle Regeneration Following Trauma
| Autor: | Peter Genovese, Andrew J. Dunn, Genevieve Hilliard, Koyal Garg, Jeffrey Au, Josh Madsen, Hannah Chauvin, Allison Paoli, Gabriel Haas, Charles West, Natalia Ziemkiewicz |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Biomedical Engineering Bioengineering Endogeny Biochemistry Fibrin Laminin 111 Biomaterials Tissue engineering Tibialis anterior muscle Muscular Diseases Internal medicine Myosin medicine Animals Humans Regeneration Muscle Skeletal biology Chemistry Skeletal muscle Hydrogels Rats Endocrinology medicine.anatomical_structure Self-healing hydrogels biology.protein Laminin |
| Zdroj: | Tissue engineering. Part A. 28(7-8) |
| ISSN: | 1937-335X |
| Popis: | Volumetric muscle loss (VML) is the surgical or traumatic loss of skeletal muscle which can cause loss of limb function or permanent disability. VML injuries overwhelms the endogenous regenerative capacity of skeletal muscle and results in poor functional healing outcomes. Currently, there are no approved tissue engineering treatments for VML injuries. In this study, fibrin hydrogels enriched with laminin-111 (50-450 µg/ml) were used for the treatment of VML of the tibialis anterior muscle in a rat model. Treatment with fibrin hydrogel containing 450 µg/ml of laminin-111 (FBN450) improved muscle regeneration following VML injury. FBN450 hydrogel treatment increased the relative proportion of contractile to fibrotic tissue as indicated by the myosin: collagen ratio on day 28 post-VML injury. FBN450 hydrogels also enhanced myogenic protein expression and increased the quantity of small to medium size myofibers (500-2000 µm2) as well as innervated myofibers. Improved contractile tissue deposition due to FBN450 hydrogel treatment resulted in a significant improvement (~60%) in torque production at day 28 post-injury. Taken together, these results suggest that the acellular FBN450 hydrogels provide a promising therapeutic strategy for VML that is worthy of further investigation. |
| Databáze: | OpenAIRE |
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