Management of seed-borne fungi in cowpea using leaf extracts and sodium bicarbonate
Autor: | Kehinde Titilope Kareem, Adijat Yetunde Shorinmade, O.A. Akinbode, Qasim Olaitan Afolabi |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
Sodium bicarbonate biology Chemistry fungi food and beverages 04 agricultural and veterinary sciences biology.organism_classification 01 natural sciences Cowpea eco-friendly fungicide inhibition 040501 horticulture Fungicide Horticulture chemistry.chemical_compound Candida spp Rhizopus stolonifer Potato dextrose agar Pythium Cultivar 0405 other agricultural sciences Inhibitory effect 010606 plant biology & botany |
Zdroj: | Journal of Applied Sciences and Environmental Management; Vol 22, No 4 (2018); 565-570 |
ISSN: | 1119-8362 |
DOI: | 10.4314/jasem.v22i4.23 |
Popis: | The study investigated the in-vitro antifungal activities of sodium bicarbonate (NaHCO 3 ) and leaf extracts of Vernonia amydgalina (bitter leaf) and A zadirachta indica (neem) on fungal species isolated from two cowpea cultivars; IFE BROWN and Drum (a local cultivar). Concentrations of 1.95 mg/ml and 1.43 mg/ml were used for A. indica while 1.45 mg/ml and 1.15 mg/ml were used for V. amydgalina . Sodium bicarbonate was evaluated on all the isolates at 2.0 mg/ml and 3.0 mg/ml. The inhibitory effects of these antifungal agents were compared with benlate (a synthetic fungicide) on Potato Dextrose Agar. Benlate totally inhibited all the fungal isolates. Candida species isolated from the two cowpea cultivars were greatly inhibited by the antifungal agents while Trichoderma species isolated from IFE BROWN was the least inhibited. The inhibitory effects of NaHCO 3 and A. indica on Candida spp. were not significantly different and values ranged from 74.5% to 84%. The two concentrations of A. indica had better inhibitory effect on Pythium spp . than NaHCO 3 . Vernonia amydgalina at 1.45 mg/ml inhibited A. niger and Candida spp. by 83.75% and 87.5% respectively while NaHCO 3 at 2.0 mg/ml inhibited Rhizopus stolonifer isolated from Drum by 75%. The study concludes that the two plant extracts and sodium bicarbonate had broad spectrum activities on fungal species and are therefore recommended as alternatives to toxic and synthetic fungicides. Keywords: Cowpea, eco-friendly, fungicide, inhibition |
Databáze: | OpenAIRE |
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