Antisera against Periplaneta americana Cu,Zn-superoxide dismutase (SOD): separation of the neurohormone bursicon from SOD, and immunodetection of SOD in the central nervous system
| Autor: | Barbara Kostron, Josef Kellermann, Hans-Willi Honegger, D. Market, C E Carter |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Central Nervous System
Male animal structures Invertebrate Hormones Blotting Western Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Biochemistry Immunoenzyme Techniques Superoxide dismutase Antibody Specificity Animals Periplaneta Amino Acid Sequence Molecular Biology Peptide sequence Chromatography High Pressure Liquid Bursicon chemistry.chemical_classification biology Superoxide Dismutase fungi biology.organism_classification Molecular biology Amino acid Drosophila virilis chemistry Insect Science Ventral nerve cord biology.protein Female Rabbits Invertebrate hormone |
| Zdroj: | Insect Biochemistry and Molecular Biology. 29:861-871 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/s0965-1748(99)00060-0 |
| Popis: | In an effort to characterize the insect molting hormone bursicon from the cockroach, Periplaneta americana, amino acid sequences with high identity of Cu,Zn-superoxide dismutase (SOD) of Drosophila virilis were identified. Antisera against a conserved region of SOD, and a sequence unique to Periplaneta SOD were produced and used to test whether bursicon might be a form of SOD. Western blots of one- and two-dimensional gels revealed that the dimeric form of SOD and bursicon have a similar molecular mass (30 kDa). The two proteins can be separated, however, according to their different isoelectric points. Bursicon is identified in two-dimensional gels by elution from four unique spots not labeled by the anti-SOD antisera. In sections of Periplaneta nerve cords the antisera labeled glial material surrounding neuronal somata close to the neural sheath. Bursicon, however, is contained in unique cell pairs in the ganglia of the ventral nerve cord. These neurons were labeled with new antisera produced against novel sequences of one of the four above-mentioned bursicon active spots. The results show unequivocally that SOD and bursicon are distinctly different proteins. Furthermore, the anti-SOD antisera provided a tool to isolate and sequence bursicon. |
| Databáze: | OpenAIRE |
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