Analysis of Cellular EMT States Using Molecular Biology and High Resolution FISH Labeling
| Autor: | Kerstin Bystricky, Noemie Kempf, Anne-Claire Lavigne, Sylvain Cantaloube, Isabelle Goiffon, Fatima Moutahir |
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| Přispěvatelé: | Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2020 |
| Předmět: |
Hybrid EMT
Plasticity [SDV]Life Sciences [q-bio] Vimentin Biology Metastasis 03 medical and health sciences 0302 clinical medicine DNA-FISH Epithelial–mesenchymal transition ComputingMilieux_MISCELLANEOUS Cancer 030304 developmental biology 0303 health sciences Genome Cadherin High-throughput microscopy Mesenchymal stem cell Chromatin reorganization Epithelial-mesenchymal transition Phenotype 3. Good health Chromatin Cell biology 030220 oncology & carcinogenesis Cancer cell biology.protein Reprogramming |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071607787 Analysis of cellular EMT states usingmolecular biology and high resolution FISH labelling Analysis of cellular EMT states usingmolecular biology and high resolution FISH labelling, pp.353-383, 2021, ⟨10.1007/978-1-0716-0779-4_27⟩ Methods Mol Biol. Methods Mol Biol., 2179, pp.353-383, 2021, ⟨10.1007/978-1-0716-0779-4_27⟩ |
| DOI: | 10.1007/978-1-0716-0779-4_27 |
| Popis: | International audience; Metastasis results from the ability of cancer cells to grow and to spread beyond the primary tumor to distant organs. Epithelial-to-Mesenchymal Transition (EMT), a fundamental developmental process, is reactivated in cancer cells, and causes epithelial properties to evolve into mesenchymal and invasive ones. EMT changes cellular characteristics between two distinct states, yet, the process is not binary but rather reflects a broad spectrum of partial EMT states in which cells exhibit various degrees of intermediate epithelial and mesenchymal phenotypes. EMT is a complex multistep process that involves cellular reprogramming through numerous signaling pathways, alterations in gene expression, and changes in chromatin morphology. Therefore, expression of key proteins, including cadherins, occludin, or vimentin must be precisely regulated. A comprehensive understanding of how changes in nuclear organization, at the level of single genes clusters, correlates with these processes during formation of metastatic cells is still missing and yet may help personalized prognosis and treatment in the clinic. Here, we describe methods to correlate physiological and molecular states of cells undergoing an EMT process with chromatin rearrangements observed via FISH labeling of specific domains. |
| Databáze: | OpenAIRE |
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