| Autor: |
Nadine Goelz, Anissja Karer, Svantje Tauber, Anna Wang, Claudia Dumrese, Oliver Ullrich, Durie Suh, Sandra Hunziker, Maria Birlem, Fengyuan Zhuang, Chen Sang, Stephanie Engeli, Dana Michaela Simmet, Katrin Paulsen, Josefine Biskup, Eva Hürlimann, Christoph Signer, Shalimar Konopasek, Karl-Heinrich Grote |
| Jazyk: |
angličtina |
| Předmět: |
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| Zdroj: |
Acta Astronautica. (1):277-292 |
| ISSN: |
0094-5765 |
| DOI: |
10.1016/j.actaastro.2013.06.007 |
| Popis: |
During spaceflight the immune system is one of the most affected systems of the human body. During the SIMBOX (Science in Microgravity Box) mission on Shenzhou-8, we investigated microgravity-associated long-term alterations in macrophageal cells, the most important effector cells of the immune system. We analyzed the effect of long-term microgravity on the cytoskeleton and immunologically relevant surface molecules. Human U937 cells were differentiated into a macrophageal phenotype and exposed to microgravity or 1 g on a reference centrifuge on-orbit for 5 days. After on-orbit fixation, the samples were analyzed with immunocytochemical staining and confocal microscopy after landing. The unmanned Shenzhou-8 spacecraft was launched on board a Long March 2F (CZ-2F) rocket from the Jiuquan Satellite Launch Center (JSLC) and landed after a 17-day-mission. We found a severely disturbed actin cytoskeleton, disorganized tubulin and distinctly reduced expression of CD18, CD36 and MHC-II after the 5 days in microgravity. The disturbed cytoskeleton, the loss of surface receptors for bacteria recognition, the activation of T lymphocytes, the loss of an important scavenger receptor and of antigen-presenting molecules could represent a dysfunctional macrophage phenotype. This phenotype in microgravity would be not capable of migrating or recognizing and attacking pathogens, and it would no longer activate the specific immune system, which could be investigated in functional assays. Obviously, the results have to be interpreted with caution as the model system has some limitations and due to numerous technical and biological restrictions (e.g. 23 °C and no CO 2 supply during in-flight incubation). All parameter were carefully pre-tested on ground. Therefore, the experiment could be adapted to the experimental conditions available on Shenzhou-8. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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