Histological and immunohistochemical changes in the rat oral mucosa used as an autologous urethral graft
| Autor: | Antonio M. Ruiz-Montes, Esther Liceras, MA Martín-Piedra, Fatima Martín-Cano, Miguel Alaminos, Carolina Marañés, Ingrid Garzón, Ricardo Fernández-Valadés |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty Urethroplasty medicine.medical_treatment Immunofluorescence Cytokeratin Urethra medicine Animals Rats Wistar Oral mucosa medicine.diagnostic_test business.industry Transdifferentiation Mouth Mucosa General Medicine Immunohistochemistry Epithelium Rats medicine.anatomical_structure Pediatrics Perinatology and Child Health Surgery business |
| Zdroj: | Journal of Pediatric Surgery. 48:1557-1564 |
| ISSN: | 0022-3468 |
| DOI: | 10.1016/j.jpedsurg.2013.01.056 |
| Popis: | Purpose The purpose of this study was to determine the histological and functional (immunohistochemical) changes that take place in oral mucosa grafts implanted in the rat urethra. Methods Urethroplasty was performed in 26 male Wistar rats weighing 250 g. All animals received autologous oral mucosa urethra grafting under general anesthesia. Samples were analyzed 10, 20, 30, 40, 50, 60, 90, and 120 days after surgery using light and scanning electron microscopy and immunofluorescence for the determination of the expression of epithelial markers (pancytokeratin, cytokeratin 1, 4, 13, and filaggrin). Results Grafted oral mucosa tissues were subjected to significant histological changes from the beginning with the formation of a well-developed epithelium whose structure was comparable to the native urethra from day 60 of the surgical implant. The immunofluorescence analysis demonstrated that the cytokeratin expression profile tended to mimic the pattern of the native urethra. These data suggest that the oral mucosa is able to efficiently transdifferentiate to the urethral environment. Conclusions The efficient transdifferentiation process of the grafted oral mucosa at both the histological and immunofluorescence levels, and the absence of local complications confirm the clinical usefulness of this type of tissues for the repair of the urethra. |
| Databáze: | OpenAIRE |
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